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Escherichia coli K-12 substr. MG1655 Transporter: gluconate / fructuronate transporter GntP



Gene: gntP Accession Numbers: EG12563 (EcoCyc), b4321, ECK4312

Synonyms: yjiB

Regulation Summary Diagram: ?

Summary:
GntP is a member of the GntP family transporters [Peekhaus97] and is homologous to the E. coli GntT and GntU gluconate transporters. GntP transport D-fructuronate/D-gluconate [Klemm96, Bates04]. Whole cell experiments have shown that the cloned gntP gene confers gluconate transport with a Km of approx 25 μM, but its expression is not induced by gluconate [Klemm96]. gntP gene is induced by fructuronate, the first intermediate of the glucuronate metabolism pathway. It is a member of UxuR regulon which is composed of genes induced by glucuronate via its conversion to fructuronate and these genes are repressed by UxuR [RobertBaudouy81]. gntP mutants were unable to grow using fructuronate as their sole carbon source. Taken together, these results indicate that GntP is the primary fructuronate transporter [Bates04].

gntP was predicted to be a target of the small RNA OmrB. Overexpression of both OmrB and OmrA decreases the expression of gntP [Tjaden06].

Locations: inner membrane

Map Position: [4,547,976 <- 4,549,319] (98.02 centisomes)
Length: 1344 bp / 447 aa

Molecular Weight of Polypeptide: 47.138 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014161 , EchoBASE:EB2451 , EcoGene:EG12563 , EcoliWiki:b4321 , OU-Microarray:b4321 , PortEco:gntP , Protein Model Portal:P0AC94 , RefSeq:NP_418741 , RegulonDB:EG12563 , String:511145.b4321 , UniProt:P0AC94

Relationship Links: InterPro:IN-FAMILY:IPR003474 , Pfam:IN-FAMILY:PF02447

In Paralogous Gene Group: 17 (37 members) , 469 (2 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0015992 - proton transport Inferred from experiment [Bates04]
GO:0035429 - gluconate transmembrane transport Inferred from experiment Inferred by computational analysis [GOA01, Klemm96]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0019521 - D-gluconate metabolic process Inferred by computational analysis [UniProtGOA11]
GO:0046177 - D-gluconate catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0005351 - sugar:proton symporter activity Inferred from experiment [Bates04]
GO:0015128 - gluconate transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA01, Klemm96]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Hsieh10a]
GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Klemm96]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for gntP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: D-fructuronate transporter (gluconate / fructuronate transporter GntP)


Enzymatic reaction of: D-gluconate transprter (gluconate / fructuronate transporter GntP)

Synonyms: Transport of gluconate


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 1 -> 21
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 29 -> 49
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 55 -> 75
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 63
[Klemm96, UniProt10]
Alternate sequence: E → A; UniProt: (in Ref. 1; CAA62859);
Transmembrane-Region 104 -> 124
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 127 -> 147
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 176 -> 196
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 225 -> 245
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 261 -> 281
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 295 -> 315
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 330 -> 350
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 352 -> 372
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 375 -> 395
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 427 -> 447
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b4321 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12563; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bates04: Bates Utz C, Nguyen AB, Smalley DJ, Anderson AB, Conway T (2004). "GntP is the Escherichia coli Fructuronic acid transporter and belongs to the UxuR regulon." J Bacteriol 186(22);7690-6. PMID: 15516583

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hsieh10a: Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J (2010). "Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini." Protein Sci 19(4);868-80. PMID: 20196076

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Klemm96: Klemm P, Tong S, Nielsen H, Conway T (1996). "The gntP gene of Escherichia coli involved in gluconate uptake." J Bacteriol 178(1);61-7. PMID: 8550444

Peekhaus97: Peekhaus N, Tong S, Reizer J, Saier MH, Murray E, Conway T (1997). "Characterization of a novel transporter family that includes multiple Escherichia coli gluconate transporters and their homologues." FEMS Microbiol Lett 1997;147(2);233-8. PMID: 9119199

RobertBaudouy81: Robert-Baudouy J, Portalier R, Stoeber F (1981). "Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products." J Bacteriol 145(1);211-20. PMID: 7007313

Tjaden06: Tjaden B, Goodwin SS, Opdyke JA, Guillier M, Fu DX, Gottesman S, Storz G (2006). "Target prediction for small, noncoding RNAs in bacteria." Nucleic Acids Res 34(9);2791-802. PMID: 16717284

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Other References Related to Gene Regulation

Blanco86a: Blanco C, Ritzenthaler P, Kolb A (1986). "The regulatory region of the uxuAB operon in Escherichia coli K12." Mol Gen Genet 1986;202(1);112-9. PMID: 3083215

Ritzenthaler82: Ritzenthaler P, Mata-Gilsinger M (1982). "Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression." J Bacteriol 150(3);1040-7. PMID: 6281232

Rodionov00: Rodionov DA, Mironov AA, Rakhmaninova AB, Gelfand MS (2000). "Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria." Mol Microbiol 38(4);673-83. PMID: 11115104

Zheng01: Zheng M, Wang X, Templeton LJ, Smulski DR, LaRossa RA, Storz G (2001). "DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide." J Bacteriol 183(15);4562-70. PMID: 11443091

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc11.