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Escherichia coli K-12 substr. MG1655 Transporter: serine / threonine:Na+ symporter



Gene: sstT Accession Numbers: EG12732 (EcoCyc), b3089, ECK3080

Synonyms: ygjU, SstT DAACS transporter

Regulation Summary Diagram: ?

Summary:
The YgjU/SstT protein is a member of the DAACS family of sodium ion coupled serine/threonine symporters. The sstT gene has been cloned and shown to complement a serine transport mutant [Ogawa98].

The SstT protein has been purified and demonstrated in reconstituted proteoliposomes to mediate the transport of serine driven by electrochemical sodium ion gradient with a Km and Vmax of 0.82 μM and 0.37 nmol/min/mg protein respectively. Serine transport was inhibited by L-threonine, but not by other amino acids [Kim02].

Locations: inner membrane

Map Position: [3,237,966 -> 3,239,210] (69.79 centisomes)
Length: 1245 bp / 414 aa

Molecular Weight of Polypeptide: 43.478 kD (from nucleotide sequence), 43.5 kD (experimental) [Ogawa98 ]

Unification Links: ASAP:ABE-0010159 , EchoBASE:EB2590 , EcoGene:EG12732 , EcoliWiki:b3089 , OU-Microarray:b3089 , PortEco:sstT , PR:PRO_000023994 , Protein Model Portal:P0AGE4 , RefSeq:NP_417560 , RegulonDB:EG12732 , SMR:P0AGE4 , String:511145.b3089 , UniProt:P0AGE4

Relationship Links: InterPro:IN-FAMILY:IPR001991 , InterPro:IN-FAMILY:IPR023025 , Panther:IN-FAMILY:PTHR11958 , Pfam:IN-FAMILY:PF00375 , Prints:IN-FAMILY:PR00173 , Prosite:IN-FAMILY:PS00713 , Prosite:IN-FAMILY:PS00714

In Paralogous Gene Group: 488 (3 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006865 - amino acid transport Inferred from experiment Inferred by computational analysis [UniProtGOA11, Kim02]
GO:0032329 - serine transport Inferred from experiment Inferred by computational analysis [GOA01, Kim02]
GO:0003333 - amino acid transmembrane transport Inferred by computational analysis [GOA06]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006835 - dicarboxylic acid transport Inferred by computational analysis [GOA01]
GO:0015826 - threonine transport Inferred by computational analysis [GOA01]
GO:0055085 - transmembrane transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005295 - neutral amino acid:sodium symporter activity Inferred from experiment [Kim02]
GO:0015175 - neutral amino acid transmembrane transporter activity Inferred from experiment [Kim02]
GO:0015171 - amino acid transmembrane transporter activity Inferred by computational analysis [GOA06]
GO:0015293 - symporter activity Inferred by computational analysis [UniProtGOA11, GOA06]
GO:0015565 - threonine efflux transmembrane transporter activity Inferred by computational analysis [GOA01]
GO:0017153 - sodium:dicarboxylate symporter activity Inferred by computational analysis [GOA01]
GO:0022889 - serine transmembrane transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, DiazMejia09, Daley05]
GO:0016021 - integral component of membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, Kim02]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids serine
metabolism biosynthesis of building blocks amino acids threonine
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for sstT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: serine / threonine:Na+ symporter

Synonyms: transport of L-serine


Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Kim02, UniProt11a]
UniProt: Removed.
Chain 2 -> 414
[UniProt09]
UniProt: Serine/threonine transporter sstT;
Transmembrane-Region 16 -> 36
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 46 -> 66
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 84 -> 104
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 143 -> 163
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 180 -> 200
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 218 -> 238
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 300 -> 320
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 332 -> 352
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3089 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12732; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kim02: Kim YM, Ogawa W, Tamai E, Kuroda T, Mizushima T, Tsuchiya T (2002). "Purification, reconstitution, and characterization of Na(+)/serine symporter, SstT, of Escherichia coli." J Biochem (Tokyo) 132(1);71-6. PMID: 12097162

Ogawa98: Ogawa W, Kim YM, Mizushima T, Tsuchiya T (1998). "Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter." J Bacteriol 180(24);6749-52. PMID: 9852024

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

Pulvermacher09: Pulvermacher SC, Stauffer LT, Stauffer GV (2009). "The Small RNA GcvB Regulates sstT mRNA Expression in Escherichia coli." J Bacteriol 191(1):238-48. PMID: 18952787


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC14B.