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Escherichia coli K-12 substr. MG1655 Transporter: K+ : H+ antiporter KefB



Gene: kefB Accession Numbers: EG20110 (EcoCyc), b3350, ECK3338

Synonyms: trkB, KefB potassium CPA2 transporter

Regulation Summary Diagram: ?

Summary:
KefB and KefC are two independent glutathione-regulated potassium efflux systems, which play a role in protecting the cell from electrophile toxicity. Mutations in kefB and kefC affect potassium efflux at neutral pH, can be complemented by the cloned genes and probably function via potassium/proton antiport [Booth85, Bakker87]. Potassium efflux by KefB or KefC is activated by adducts formed by reaction of glutathione with electrophilic compounds such as N-ethylmaleimide, methylglyoxal and chlorodinitrobenzene [Ferguson93, Elmore90]. Potassium efflux mediated by KefB and KefC leads to acidification of the cytoplasm, which protects the cell from electrophile toxicity [Ferguson97a, Ferguson95].

KefB and KefC differ in their activation by methylglyoxal, with KefC only weakly activated [Ness99]. KefB is a member of the CPA2 family of monovalent cation/proton antiporters.

In addition to KefB and KefC, additional unidentified potassium efflux systems exist.

Locations: inner membrane

Map Position: [3,476,824 <- 3,478,629] (74.94 centisomes)
Length: 1806 bp / 601 aa

Molecular Weight of Polypeptide: 66.411 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010949 , CGSC:83 , EchoBASE:EB4144 , EcoGene:EG20110 , EcoliWiki:b3350 , ModBase:P45522 , OU-Microarray:b3350 , PortEco:kefB , PR:PRO_000023061 , Pride:P45522 , Protein Model Portal:P45522 , RefSeq:NP_417809 , RegulonDB:EG20110 , SMR:P45522 , String:511145.b3350 , UniProt:P45522

Relationship Links: InterPro:IN-FAMILY:IPR003148 , InterPro:IN-FAMILY:IPR004771 , InterPro:IN-FAMILY:IPR006153 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR020884 , Pfam:IN-FAMILY:PF00999 , Pfam:IN-FAMILY:PF02254 , Prosite:IN-FAMILY:PS51201

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006813 - potassium ion transport Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Bakker87]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11]
GO:0006812 - cation transport Inferred by computational analysis [GOA01]
GO:0071805 - potassium ion transmembrane transport Inferred by computational analysis [GOA06, GOA01]
GO:0098655 - cation transmembrane transport Inferred by computational analysis [GOA01]
GO:1902600 - hydrogen ion transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0008324 - cation transmembrane transporter activity Inferred by computational analysis [GOA01]
GO:0015297 - antiporter activity Inferred by computational analysis [UniProtGOA11]
GO:0015299 - solute:proton antiporter activity Inferred by computational analysis [GOA01]
GO:0015503 - glutathione-regulated potassium exporter activity Inferred by computational analysis [GOA06, GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA01, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [GOA06]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for kefB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: K+ : H+ antiporter KefB


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 4 -> 24
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 29 -> 49
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 55 -> 75
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 87 -> 107
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 115 -> 135
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 152 -> 172
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 177 -> 197
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 207 -> 227
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 230 -> 250
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 268 -> 288
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 291 -> 311
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 324 -> 344
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Transmembrane-Region 356 -> 376
[UniProt13]
UniProt: Helical; Non-Experimental Qualifier: potential.
Conserved-Region 402 -> 524
[UniProt09]
UniProt: RCK N-terminal;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3350 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG20110; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bakker87: Bakker EP, Booth IR, Dinnbier U, Epstein W, Gajewska A (1987). "Evidence for multiple K+ export systems in Escherichia coli." J Bacteriol 1987;169(8);3743-9. PMID: 3301813

Booth85: Booth IR, Epstein W, Giffard PM, Rowland GC (1985). "Roles of the trkB and trkC gene products of Escherichia coli in K+ transport." Biochimie 1985;67(1);83-9. PMID: 3888294

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Elmore90: Elmore MJ, Lamb AJ, Ritchie GY, Douglas RM, Munro A, Gajewska A, Booth IR (1990). "Activation of potassium efflux from Escherichia coli by glutathione metabolites." Mol Microbiol 1990;4(3);405-12. PMID: 2192231

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Ferguson93: Ferguson GP, Munro AW, Douglas RM, McLaggan D, Booth IR (1993). "Activation of potassium channels during metabolite detoxification in Escherichia coli." Mol Microbiol 1993;9(6);1297-303. PMID: 7934942

Ferguson95: Ferguson GP, McLaggan D, Booth IR (1995). "Potassium channel activation by glutathione-S-conjugates in Escherichia coli: protection against methylglyoxal is mediated by cytoplasmic acidification." Mol Microbiol 1995;17(6);1025-33. PMID: 8594323

Ferguson97a: Ferguson GP, Nikolaev Y, McLaggan D, Maclean M, Booth IR (1997). "Survival during exposure to the electrophilic reagent N-ethylmaleimide in Escherichia coli: role of KefB and KefC potassium channels." J Bacteriol 1997;179(4);1007-12. PMID: 9023177

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ness99: Ness LS, Booth IR (1999). "Different foci for the regulation of the activity of the KefB and KefC glutathione-gated K+ efflux systems." J Biol Chem 1999;274(14);9524-30. PMID: 10092637

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13B.