Escherichia coli K-12 substr. MG1655 tRNA: tRNAleuW

Gene: leuW Accession Numbers: EG30052 (EcoCyc), b0672, ECK0660

Synonyms: feeB

Superclasses: a tRNAleu

Regulation Summary Diagram: ?

Regulation summary diagram for leuW

tRNA(leuW) is one of eight leucine tRNAs.

tRNAs are the adapters that allow synthesis of proteins from mRNAs. Each tRNA carries a specific amino acid to the ribosome for protein synthesis. There, the tRNA recognizes an RNA codon with its own three-nucleotide anticodon, thus allowing synthesis of a specific peptide based on an mRNA template.

tRNAs are processed to their active, mature forms by RNA cleavage and by modification of their bases. RNA cleavage consists of removal of both 5' and 3' extensions in a multistep process involving many RNases [Morl01]. RNases taking part in tRNA processing include ribonuclease E, RNase BN, RNase D, ribonuclease II, and RNase T. tRNAs are also subject to a wide variety of base modifications catalyzed by proteins such as tRNA-dihydrouridine synthase A, tRNA(i6A37) synthase, isopentenyl-adenosine A37 tRNA methylthiolase, tRNA-specific 2-thiouridylase, fused 5-methylaminomethyl-2-thiouridine-forming methyltransferase and FAD-dependent demodification enzyme, tRNA-guanine transglycosylase, tRNA m7G46 methyltransferase, tRNA pseudouridine 13 synthase, tRNA pseudouridine 65 synthase, tRNA pseudouridine 55 synthase, tRNA pseudouridine synthase I, tRNA (Gm18) 2'-O-methyltransferase, and tRNA m5U54 methyltransferase.

Mature tRNAs are linked via a 3' CCA sequence to their cognate amino acid in an ATP-dependent fashion by the appropriate amino-acid-tRNA synthetase, as shown in the tRNA charging. Subsequently, these charged tRNAs interact with the ribosome and template mRNA to generate polypeptides. The discovery of the role of tRNA in protein synthesis is reviewed in detail in [Siekevitz81].

tRNA(leuW) can read the CUU codon that is also read by tRNA(leuU) due to a uridine-5-oxyacetic acid modification [Sorensen05].

Mutations that alter base 77 from C to A or that simply reduce tRNA(leuW) expression generally confer resistance to a calmodulin inhibitor that acts to inhibit cell division [Chen91b, Bouquin96].

Map Position: [696,186 <- 696,270] (15.01 centisomes, 54°)
Length: 85 bp

Anticodon: UAG

Reactions known to consume the compound:

tRNA charging :
a tRNAleu + L-leucine + ATP + H+ → an L-leucyl-[tRNAleu] + AMP + diphosphate

Reactions known to produce the compound:

tRNA processing :
a tRNA precursor with a short 3' extension → an uncharged tRNA + n a nucleoside 5'-monophosphate
a tRNA precursor with a short 3' extension + n phosphate → an uncharged tRNA + n a ribonucleoside diphosphate
a tRNA precursor with a 5' extension + H2O → an uncharged tRNA + a single-stranded RNA

Not in pathways:
an N-modified aminoacyl-[tRNA] + H2O → an uncharged tRNA + an N-modified amino acid + 2 H+
a D-aminoacyl-[tRNA] + H2O → a D-amino acid + an uncharged tRNA + 2 H+

Not in pathways:
a tRNA precursor + H2O → a tRNA + a nucleoside 5'-monophosphate

tRNA processing :
a tRNA precursor with a 5' extension and a short 3' extension + H2O → a tRNA precursor with a short 3' extension + a single-stranded RNA
a tRNA precursor with a 5' extension + H2O → an uncharged tRNA + a single-stranded RNA

Not in pathways:
YhaV endonuclease degradation substrate mRNA + H2O → 2 a single-stranded RNA
an mRNA + H2O → a single-stranded RNA + a single-stranded RNA
an mRNA + H2O → a single-stranded RNA + a single-stranded RNA
RNase E degradation substrate mRNA + n H2O → n a single-stranded RNA
YhaV endonuclease degradation substrate rRNA + H2O → 2 a single-stranded RNA
RNase III mRNA processing substrate + 2 H2O → RNase III processing product mRNA + 2 a single-stranded RNA
23S rRNA[periplasmic space] + H2O[periplasmic space] → 2 a single-stranded RNA[periplasmic space]
an mRNA[periplasmic space] + H2O[periplasmic space] → 2 a single-stranded RNA[periplasmic space]
RNase G degradation substrate mRNA + H2O → 2 a single-stranded RNA
9S rRNA + 2 H2O → 5S rRNA + 2 a single-stranded RNA
RNase E mRNA processing substrate + n H2O → RNase E processing product mRNA + n a single-stranded RNA

Reactions known to both consume and produce the compound:

Not in pathways:
a single-stranded RNA + phosphate ↔ a single-stranded RNA + a nucleoside diphosphate

In Reactions of unknown directionality:

Not in pathways:
an L-leucyl-[tRNAleu] + a protein = a tRNAleu + L-leucyl-[protein] + H+

Not in pathways:
rRNA[periplasmic space] = 2 a single-stranded RNA[periplasmic space]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for leuW

Unification Links: ASAP:ABE-0002288 , CGSC:562 , EchoBASE:EB4215 , EcoGene:EG30052 , EcoliWiki:b0672 , OU-Microarray:b0672 , PortEco:leuW , RegulonDB:EG30052

GO Terms:

Molecular Function: GO:0030533 - triplet codon-amino acid adaptor activity
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol

MultiFun Terms: information transfer RNA related tRNA

Transcription Units regulated by related protein L-leucyl-tRNAleuW (4 total):

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0672 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG30052.

Last-Curated ? 26-Apr-2006 by Shearer A , SRI International


Bouquin96: Bouquin N, Chen MX, Kim S, Vannier F, Bernard S, Holland IB, Seror SJ (1996). "Characterization of an Escherichia coli mutant, feeA, displaying resistance to the calmodulin inhibitor 48/80 and reduced expression of the rare tRNA3Leu." Mol Microbiol 20(4);853-65. PMID: 8793881

Chen91b: Chen MX, Bouquin N, Norris V, Casaregola S, Seror SJ, Holland IB (1991). "A single base change in the acceptor stem of tRNA(3Leu) confers resistance upon Escherichia coli to the calmodulin inhibitor, 48/80." EMBO J 10(10);3113-22. PMID: 1915285

Morl01: Morl M, Marchfelder A (2001). "The final cut. The importance of tRNA 3'-processing." EMBO Rep 2(1);17-20. PMID: 11252717

Siekevitz81: Siekevitz P, Zamecnik PC (1981). "Ribosomes and protein synthesis." J Cell Biol 91(3 Pt 2);53s-65s. PMID: 7033244

Sorensen05: Sorensen MA, Elf J, Bouakaz E, Tenson T, Sanyal S, Bjork GR, Ehrenberg M (2005). "Over expression of a tRNA(Leu) isoacceptor changes charging pattern of leucine tRNAs and reveals new codon reading." J Mol Biol 354(1);16-24. PMID: 16236318

Other References Related to Gene Regulation

Mohanty08: Mohanty BK, Kushner SR (2008). "Rho-independent transcription terminators inhibit RNase P processing of the secG leuU and metT tRNA polycistronic transcripts in Escherichia coli." Nucleic Acids Res 36(2);364-75. PMID: 18033800

Nakajima81: Nakajima N, Ozeki H, Shimura Y (1981). "Organization and structure of an E. coli tRNA operon containing seven tRNA genes." Cell 1981;23(1);239-49. PMID: 6163550

Nakajima82: Nakajima N, Ozeki H, Shimura Y (1982). "In vitro transcription of the supB-E tRNA operon of Escherichia coli. Characterization of transcription products." J Biol Chem 1982;257(18);11113-20. PMID: 6286682

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Apr 1, 2015, BIOCYC13B.