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Escherichia coli K-12 substr. MG1655 Polypeptide: toxin of the GhoTS toxin-antitoxin system



Gene: ghoT Accession Numbers: G0-10472 (EcoCyc), b4559, ECK4122

Synonyms: yjdO

Regulation Summary Diagram: ?

Summary:
GhoT is the toxin of a novel type V toxin-antitoxin system. GhoT is a peptide that damages the membrane, reduces cellular ATP levels and disrupts membrane potential, causing ghost cell formation and increasing persistence [Wang12b, Cheng14]. The GhoS antitoxin acts by specifically cleaving ghoT mRNA [Wang12b].

ghoT is one of a small set of coding regions that do not contain cleavage sites for the MqsR toxin, whereas the ghoS coding region contains three consensus MqsR cleavage sites. Thus, in the presence of MqsR, the ghoS-encoding 5' region of the ghoST transcript is degraded, thereby enriching for the ghoT-encoding 3' region [Wang13b].

A ghoT deletion mutant shows decreased MqsR-mediated persister cell formation [Wang12b]. An F38R mutant maintains polar localization, but is no longer toxic [Cheng14].

Phenotype microarray experiments using scarless ΔghoST and ghoT mutants showed differential response to a set of antimicrobial compounds [Cheng14].

GhoT: "toxin producing ghost cells" [Wang12b]

Comment: [Van12]

Locations: inner membrane

Map Position: [4,350,931 -> 4,351,104] (93.78 centisomes)
Length: 174 bp / 57 aa

Molecular Weight of Polypeptide: 6.555 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0285080 , EchoBASE:EB4088 , EcoGene:EG14342 , EcoliWiki:b4559 , PortEco:yjdO , Protein Model Portal:P64646 , RefSeq:YP_588474 , RegulonDB:G0-10472 , String:511145.b4559 , UniProt:P64646

Relationship Links: InterPro:IN-FAMILY:IPR019689 , Pfam:IN-FAMILY:PF10753

GO Terms:

Biological Process: GO:0008219 - cell death Inferred from experiment [Wang12b]
Cellular Component: GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Cheng14]
GO:0060187 - cell pole Inferred from experiment [Cheng14]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes

Essentiality data for ghoT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 09-Jan-2014 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 7 -> 27
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 37 -> 57
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Thu Jan 19, 2006:
Existence of this gene was inferred by analysis performed in the 2005 E. coli annotation update [Riley06 ].


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cheng14: Cheng HY, Soo VW, Islam S, McAnulty MJ, Benedik MJ, Wood TK (2014). "Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress." Environ Microbiol 16(6);1741-54. PMID: 24373067

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Van12: Van Melderen L (2012). "GhoSTly bacterial persisters." Nat Chem Biol 8(10);812-3. PMID: 22987009

Wang12b: Wang X, Lord DM, Cheng HY, Osbourne DO, Hong SH, Sanchez-Torres V, Quiroga C, Zheng K, Herrmann T, Peti W, Benedik MJ, Page R, Wood TK (2012). "A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS." Nat Chem Biol 8(10);855-61. PMID: 22941047

Wang13b: Wang X, Lord DM, Hong SH, Peti W, Benedik MJ, Page R, Wood TK (2013). "Type II toxin/antitoxin MqsR/MqsA controls type V toxin/antitoxin GhoT/GhoS." Environ Microbiol 15(6);1734-44. PMID: 23289863


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc12.