Escherichia coli K-12 substr. MG1655 Polypeptide: MatA DNA-binding transcriptional dual regulator

Gene: ecpR Accession Numbers: G6165 (EcoCyc), b0294, ECK0293

Synonyms: ykgK, matA

Regulation Summary Diagram: ?

Regulation summary diagram for ecpR

MatA is a LuxR-type transcription factor that is directly involved, as a positive regulator acting at transcriptional and posttranscriptional levels, in the expression of the mat operon in meningitis isolate strain IHE 3034 (T. A. Lehti, P. Bauchart, M. Kukkonen, U. Dobrindt, T. K. Korhonen, B. Westerlund-Wikström, unpublished results) [Lehti12]. On the other hand, MatA controls the expression of the flhDC operon, with higher affinity to flhDp, repressing flagellum biosynthesis, motility, and taxis [Lehti12].

The opposite regulatory actions of MatA on mat and on flhDC promoters advance the adaptation of E. coli from a planktonic to an adhesive lifestyle [Lehti12]. MatA binds more weakly to pfliA and to pfliC, and their levels are equal to those observed for matB [Lehti12].

MatA exerts a dual regulatory function on the choice of planktonic/sessile lifestyle and further supports the presence of antagonistic control of attachment and motility. MatA is tightly integrated into two separate regulatory circuits, H-NS-MatA-flhDC and RcsB-MatA-flhDC, both of which control expression of the flagellum (T. A. Lehti, P. Bauchart, M. Kukkonen, U. Dobrindt, T. K. Korhonen, B. Westerlund-Wikström, unpublished results; T. A. Lehti, J. Heikkinen, T. K. Korhonen, B. Westerlund-Wikström, unpublished results).

MatA interferes with bacterial motility and flagellar synthesis in meningitis-associated isolate IHE 3034 as well as K-12 strains of Escherichia coli [Lehti12].

Locations: cytosol

Map Position: [309,970 <- 310,560] (6.68 centisomes, 24°)
Length: 591 bp / 196 aa

Molecular Weight of Polypeptide: 23.274 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001011 , EchoBASE:EB4070 , EcoGene:EG14324 , EcoliWiki:b0294 , ModBase:P71301 , OU-Microarray:b0294 , PortEco:matA , Pride:P71301 , Protein Model Portal:P71301 , RefSeq:NP_414828 , RegulonDB:G6165 , SMR:P71301 , String:511145.b0294 , UniProt:P71301

Relationship Links: InterPro:IN-FAMILY:IPR000792 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016032 , Pfam:IN-FAMILY:PF00196 , Prints:IN-FAMILY:PR00038 , Prosite:IN-FAMILY:PS50043 , Smart:IN-FAMILY:SM00421

In Paralogous Gene Group: 91 (2 members)

Genetic Regulation Schematic: ?

Genetic regulation schematic for ecpR

GO Terms:

Biological Process: GO:1902021 - regulation of bacterial-type flagellum-dependent cell motility Inferred from experiment [Lehti12]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: regulation type of regulation transcriptional level

Symmetry: Inverted Repeat

Regulated Transcription Units (6 total): ?


Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Essentiality data for ecpR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Created 24-May-2012 by Santos-Zavaleta A , UNAM

Sequence Features

Protein sequence of MatA DNA-binding transcriptional dual regulator with features indicated

Feature Class Location Citations Comment
Conserved-Region 138 -> 196
UniProt: HTH luxR-type;
DNA-Binding-Region 162 -> 181
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;
Mutagenesis-Variant 179
[Lehti12, UniProt12b]
UniProt: Slightly increases motility.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lehti12: Lehti TA, Bauchart P, Dobrindt U, Korhonen TK, Westerlund-Wikstrom B (2012). "The fimbriae activator MatA switches off motility in Escherichia coli by repression of the flagellar master operon flhDC." Microbiology 158(Pt 6);1444-55. PMID: 22422754

PerezRueda04: Perez-Rueda E, Collado-Vides J, Segovia L (2004). "Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea." Comput Biol Chem 28(5-6);341-50. PMID: 15556475

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt12b: UniProt Consortium (2012). "UniProt version 2012-11 released on 2012-11-26 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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