Escherichia coli K-12 substr. MG1655 Polypeptide: periplasmic protein associated with repression of competance

Gene: ybaV Accession Numbers: G6243 (EcoCyc), b0442, ECK0436

Regulation Summary Diagram: ?

Regulation summary diagram for ybaV

ybaV was recognised in a screen designed to identify genes that are involved in repressing cell-to-cell plasmid transfer in E. coli K-12 [Matsuda12]. YbaV contains an N-terminal signal peptide and is predicted to be located in the periplasm. YbaV contains two helix-hairpin-helix motifs that are predicted to be involved in DNA binding [Matsuda12]. YbaV may sequster DNA in the periplasm and repress transformation [Matsuda12].

ybaV shows homology to comEA - an integral membrane protein required for transformation in Bacillus subtilis [Inamine95].

Locations: inner membrane, periplasmic space

Map Position: [463,161 -> 463,532] (9.98 centisomes, 36°)
Length: 372 bp / 123 aa

Molecular Weight of Polypeptide: 12.704 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001534 , EchoBASE:EB3039 , EcoGene:EG13250 , EcoliWiki:b0442 , ModBase:P0AAR8 , OU-Microarray:b0442 , PortEco:ybaV , Protein Model Portal:P0AAR8 , RefSeq:NP_414976 , RegulonDB:G6243 , SMR:P0AAR8 , String:511145.b0442 , UniProt:P0AAR8

Relationship Links: InterPro:IN-FAMILY:IPR003583 , InterPro:IN-FAMILY:IPR004509 , InterPro:IN-FAMILY:IPR010994 , Pfam:IN-FAMILY:PF00633 , Smart:IN-FAMILY:SM00278

GO Terms:

Biological Process: GO:0006281 - DNA repair Inferred by computational analysis [GOA01a]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [GOA01a, Matsuda12]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]
GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [Matsuda12]


Essentiality data for ybaV knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Curated 18-Jan-2007 by Keseler I , SRI International
Last-Curated ? 01-Jan-2013 by Mackie A , Macquarie University

Sequence Features

Protein sequence of periplasmic protein associated with repression of competance with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 25
UniProt: Non-Experimental Qualifier: potential;
Chain 26 -> 123
UniProt: Uncharacterized protein ybaV;
Conserved-Region 60 -> 90
UniProt: HhH 1;
Conserved-Region 91 -> 120
UniProt: HhH 2;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Inamine95: Inamine GS, Dubnau D (1995). "ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport." J Bacteriol 177(11);3045-51. PMID: 7768800

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Matsuda12: Matsuda A, Kurono N, Kawano C, Shirota K, Hirabayashi A, Horino M, Etchuya R, Sobue R, Sasaki Y, Miyaue S, Sekoguchi A, Sugiura C, Shibata Y, Ito M, Ando T, Maeda S (2012). "Genome-wide screen for Escherichia coli genes involved in repressing cell-to-cell transfer of non-conjugative plasmids." Biochem Biophys Res Commun 428(4);445-50. PMID: 23131563

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, BIOCYC14A.