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Escherichia coli K-12 substr. MG1655 Polypeptide: glutathione ABC transporter - periplasmic binding protein



Gene: gsiB Accession Numbers: G6430 (EcoCyc), b0830, ECK0820

Synonyms: yliB

Regulation Summary Diagram: ?

Component of: glutathione ABC transporter (summary available)

Summary:
periplasmic binding component of glutathione ABC transporter

Locations: periplasmic space

Map Position: [868,634 -> 870,172] (18.72 centisomes)
Length: 1539 bp / 512 aa

Molecular Weight of Polypeptide: 56.47 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002832 , DIP:DIP-12697N , EchoBASE:EB3246 , EcoGene:EG13473 , EcoliWiki:b0830 , ModBase:P75797 , OU-Microarray:b0830 , PortEco:gsiB , PR:PRO_000022845 , Pride:P75797 , Protein Model Portal:P75797 , RefSeq:NP_415351 , RegulonDB:G6430 , SMR:P75797 , String:511145.b0830 , UniProt:P75797

Relationship Links: InterPro:IN-FAMILY:IPR000914 , InterPro:IN-FAMILY:IPR023765 , PDB:Structure:1UQW , Pfam:IN-FAMILY:PF00496 , Prosite:IN-FAMILY:PS01040

In Paralogous Gene Group: 25 (11 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0034634 - glutathione transmembrane transporter activity Inferred from experiment [Suzuki05a]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Han13, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Essentiality data for gsiB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: glutathione ABC transporter

Subunit composition of glutathione ABC transporter = [GsiA]2[GsiB][GsiD][GsiC]
         glutathione ABC transporter - ATP binding subunit = GsiA (summary available)
         glutathione ABC transporter - periplasmic binding protein = GsiB (summary available)
         glutathione ABC transporter - membrane subunit = GsiD (summary available)
         glutathione ABC transporter - membrane subunit = GsiC (summary available)

Summary:
The GsiABCD glutathione transporter is a member of the ATP Binding Cassette (ABC) superfamily of transporters. Based on sequence similarity, GsiA is predicted to be the ATP-binding component, GsiB is predicted to be the periplasmic binding component, and GsiC and GsiD are predicted to be inner membrane components. Mutation of any component impairs the ability of E. coli to transport the glutathione molecule into the cell. Expression of the cloned genes in their respective mutants restores their ability to transport glutathione. Transport by the glutathione ABC transporter is one of two known mechanisms for salvage of glutathione excreted from the cell. The other involves hydrolysis of glutathione by γ-glutamyl transpeptidase in the periplasm to yield glutamic acid and cysteinylglycine which can be taken back into the cell [Suzuki05a].

Locations: inner membrane

GO Terms:

Biological Process: GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
Molecular Function: GO:0034634 - glutathione transmembrane transporter activity Inferred from experiment [Suzuki05a]
GO:0043492 - ATPase activity, coupled to movement of substances Inferred from experiment [Suzuki05a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment [Suzuki05a]


Enzymatic reaction of: transport of glutathione (glutathione ABC transporter)


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 26
[UniProt10a]
UniProt: Non-Experimental Qualifier: potential;
Chain 27 -> 512
[UniProt09]
UniProt: Glutathione-binding protein gsiB;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han13: Han MJ, Kim JY, Kim JA (2013). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng. PMID: 24140104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Suzuki05a: Suzuki H, Koyanagi T, Izuka S, Onishi A, Kumagai H (2005). "The yliA, -B, -C, and -D Genes of Escherichia coli K-12 Encode a Novel Glutathione Importer with an ATP-Binding Cassette." J Bacteriol 187(17);5861-7. PMID: 16109926

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc11.