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Escherichia coli K-12 substr. MG1655 Enzyme: aldose sugar dehydrogenase



Gene: yliI Accession Numbers: G6437 (EcoCyc), b0837, ECK0827

Regulation Summary Diagram: ?

Summary:
YliI is an aldose sugar dehydrogenase that requires the cofactor pyrroloquinoline quinone (PQQ) for activity [Southall06]. E. coli lacks the ability to synthesize PQQ itself [Hommes84, Matsushita97]; however, it exhibits chemotaxis towards environmental PQQ [deJonge96], and may thus use an externally supplied cofactor [Southall06]. The physiological substrate is unknown [Southall06].

YliI localizes to the the periplasm [Southall06] and is associated with the outer membrane under certain physiological conditions [Marani06]. Sequence similarity suggested that YliI may contain β-barrel structures [Zhai02]; however, the β-propeller fold of YliI may have led to a false-positive prediction of a β-barrel structure (von Heijne, pers. comm., 8/8/2006).

The crystal structure of YliI has been determined at 1.5 Å resolution [Southall06].

Locations: periplasmic space

Map Position: [877,965 -> 879,080] (18.92 centisomes)
Length: 1116 bp / 371 aa

Molecular Weight of Polypeptide: 41.054 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002852 , EchoBASE:EB3253 , EcoGene:EG13480 , EcoliWiki:b0837 , OU-Microarray:b0837 , PortEco:yliI , Protein Model Portal:P75804 , RefSeq:NP_415358 , RegulonDB:G6437 , SMR:P75804 , String:511145.b0837 , UniProt:P75804

Relationship Links: InterPro:IN-FAMILY:IPR011041 , InterPro:IN-FAMILY:IPR011042 , InterPro:IN-FAMILY:IPR012938 , PDB:Structure:2G8S , Pfam:IN-FAMILY:PF07995

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [GOA01]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005509 - calcium ion binding Inferred from experiment [Southall06]
GO:0016901 - oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor Inferred from experiment Inferred by computational analysis [GOA01, Southall06]
GO:0070968 - pyrroloquinoline quinone binding Inferred from experiment [Southall06]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
GO:0048038 - quinone binding Inferred by computational analysis [GOA01]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Southall06]

MultiFun Terms: metabolism

Essentiality data for yliI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 23-Nov-2009 by Keseler I , SRI International


Enzymatic reaction of: aldose sugar dehydrogenase

Synonyms: Asd

EC Number: 1.1.5.-

an aldose[periplasmic space] + an oxidized electron acceptor[periplasmic space] + H2O[periplasmic space] <=> an aldonate[periplasmic space] + a reduced electron acceptor[periplasmic space] + H+[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Alternative Substrates for an aldose: maltotriose [Southall06 ] , maltose [Southall06 ] , β-D-glucose [Southall06 ]

Summary:
The enzyme has promiscuous activity towards mono-, di- and tri-saccharide aldose sugars; an activity profile is available in [Southall06]. The KM towards maltose and maltotriose is 170 and 150 mM, respectively, and the optimal pH with maltose as the substrate is 8.0 [Southall06].

Cofactors or Prosthetic Groups: Ca2+ [Southall06], pyrroloquinoline quinone [Southall06]

Kinetic Parameters:

Substrate
Km (μM)
Citations
β-D-glucose
400000.0
[Southall06]

pH(opt): 8.0 [Southall06]


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 20
[UniProt10]
UniProt: Non-Experimental Qualifier: potential;
Chain 21 -> 371
[UniProt09]
UniProt: Soluble aldose sugar dehydrogenase yliI;
Amino-Acid-Sites-That-Bind 82
[UniProt10a]
UniProt: PQQ;
Active-Site 147
[UniProt10]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;
Protein-Segment 214 -> 215
[UniProt10]
UniProt: PQQ; Sequence Annotation Type: region of interest;
Metal-Binding-Site 240
[UniProt10a]
UniProt: Calcium;
Metal-Binding-Site 250
[UniProt10a]
UniProt: Calcium; via carbonyl oxygen;
Amino-Acid-Sites-That-Bind 261
[UniProt10a]
UniProt: PQQ;
Protein-Segment 312 -> 314
[UniProt10]
UniProt: PQQ; Sequence Annotation Type: region of interest;
Protein-Segment 341 -> 343
[UniProt10]
UniProt: PQQ; Sequence Annotation Type: region of interest;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

deJonge96: de Jonge R, Teixeira de Mattos MJ, Stock JB, Neijssel OM (1996). "Pyrroloquinoline quinone, a chemotactic attractant for Escherichia coli." J Bacteriol 178(4);1224-6. PMID: 8576064

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hommes84: Hommes RWJ, Postma PW, Neijssel OM, Tempest DW, Dokter P, Duine JA (1984). "Evidence for a glucose dehydrogenase apoenzyme in several strains of Escherichia coli." FEMS Microbiol Lett 24;329-333.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Marani06: Marani P, Wagner S, Baars L, Genevaux P, de Gier JW, Nilsson I, Casadio R, von Heijne G (2006). "New Escherichia coli outer membrane proteins identified through prediction and experimental verification." Protein Sci 15(4);884-9. PMID: 16522795

Matsushita97: Matsushita K, Arents JC, Bader R, Yamada M, Adachi O, Postma PW (1997). "Escherichia coli is unable to produce pyrroloquinoline quinone (PQQ)." Microbiology 143 ( Pt 10);3149-56. PMID: 9353919

Southall06: Southall SM, Doel JJ, Richardson DJ, Oubrie A (2006). "Soluble aldose sugar dehydrogenase from Escherichia coli: a highly exposed active site conferring broad substrate specificity." J Biol Chem 281(41);30650-9. PMID: 16864586

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhai02: Zhai Y, Saier MH (2002). "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes." Protein Sci 11(9);2196-207. PMID: 12192075


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.