Escherichia coli K-12 substr. MG1655 Polypeptide: predicted peptidase with chaperone function

Gene: ycaL Accession Numbers: G6470 (EcoCyc), b0909, ECK0900

Regulation Summary Diagram: ?

Regulation summary diagram for ycaL

YcaL belongs to the widely conserved M48 family of metalloproteases [Weski12].

Locations: extracellular space, membrane

Map Position: [959,487 -> 960,251] (20.68 centisomes, 74°)
Length: 765 bp / 254 aa

Molecular Weight of Polypeptide: 26.74 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003098 , DIP:DIP-11467N , EchoBASE:EB2932 , EcoGene:EG13133 , EcoliWiki:b0909 , OU-Microarray:b0909 , PortEco:ycaL , Protein Model Portal:P43674 , RefSeq:NP_415429 , RegulonDB:G6470 , SMR:P43674 , String:511145.b0909 , UniProt:P43674

Relationship Links: InterPro:IN-FAMILY:IPR001915 , Pfam:IN-FAMILY:PF01435

In Paralogous Gene Group: 422 (3 members)

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0004222 - metalloendopeptidase activity Inferred by computational analysis [GOA01a]
GO:0008233 - peptidase activity Inferred by computational analysis [UniProtGOA11a]
GO:0008237 - metallopeptidase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005576 - extracellular region Inferred by computational analysis [DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [GOA01a]

MultiFun Terms: cell processes adaptations temperature extremes

Essentiality data for ycaL knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 15-Oct-2014 by Keseler I , SRI International

Sequence Features

Protein sequence of predicted peptidase with chaperone function with features indicated

Feature Class Location Citations Comment
Metal-Binding-Site 134
UniProt: Zinc; catalytic; Non-Experimental Qualifier: potential;
Active-Site 135
UniProt: Non-Experimental Qualifier: potential;
Metal-Binding-Site 138
UniProt: Zinc; catalytic; Non-Experimental Qualifier: potential;
Metal-Binding-Site 193
UniProt: Zinc; catalytic; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 241 -> 254
[Fricke95, UniProt10a]
UniProt: (in Ref. 5; CAA58106);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fricke95: Fricke J, Neuhard J, Kelln RA, Pedersen S (1995). "The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli." J Bacteriol 1995;177(3);517-23. PMID: 7836281

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Weski12: Weski J, Ehrmann M (2012). "Genetic analysis of 15 protein folding factors and proteases of the Escherichia coli cell envelope." J Bacteriol 194(12);3225-33. PMID: 22505681

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc11.