Escherichia coli K-12 substr. MG1655 Polypeptide: periplasmic sensory protein associated with the TorRS two-component regulatory system

Gene: torT Accession Numbers: G6515 (EcoCyc), b0994, ECK0985

Synonyms: yccH

Regulation Summary Diagram: ?

Regulation summary diagram for torT

Alternative forms of periplasmic sensory protein associated with the TorRS two-component regulatory system: TorT-TMAO

TorT is a periplasmic protein involved in the regulation of the torCAD operon, whose expression is induced in the presence of TMAO. TMAO was shown to bind to TorT with a binding constant of 150 µM, promoting a conformational change of the protein. TorT interacts with the periplasmic domain of the two-component sensor protein TorS, and binding of TMAO to TorT may indirectly induce a conformational change in TorS [Baraquet06].

A torT null mutation abolishes tor operon expression; overproduction of the response regulator TorR can partially bypass the requirement for TorT [Jourlin96]. TorT is dispensable in a torS constitutive mutant [Jourlin96a].

Locations: periplasmic space

Map Position: [1,055,484 -> 1,056,512] (22.75 centisomes, 82°)
Length: 1029 bp / 342 aa

Molecular Weight of Polypeptide: 37.865 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003360 , EchoBASE:EB2500 , EcoGene:EG12616 , EcoliWiki:b0994 , ModBase:P38683 , OU-Microarray:b0994 , Pfam:PF00532 , PortEco:torT , PR:PRO_000024091 , Protein Model Portal:P38683 , RefSeq:NP_415514 , RegulonDB:G6515 , SMR:P38683 , String:511145.b0994 , UniProt:P38683

Relationship Links: InterPro:IN-FAMILY:IPR001761 , InterPro:IN-FAMILY:IPR014301 , InterPro:IN-FAMILY:IPR028082

In Paralogous Gene Group: 30 (25 members) , 581 (2 members)

In Reactions of unknown directionality:

Not in pathways:
trimethylamine N-oxide + periplasmic sensory protein associated with the TorRS two-component regulatory system = TorT-TMAO

GO Terms:

Biological Process: GO:0009061 - anaerobic respiration Inferred from experiment [Baraquet06]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [DiazMejia09, Jourlin96]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: information transfer protein related posttranslational modification
metabolism energy metabolism, carbon anaerobic respiration
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for torT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 03-Nov-2006 by Keseler I , SRI International

Sequence Features

Protein sequence of periplasmic sensory protein associated with the TorRS two-component regulatory system with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 18
Cleavage of the signal peptide was confirmed by N-terminal sequencing of the overexpressed TorT protein [Jourlin96].
Chain 19 -> 342
UniProt: Periplasmic protein torT;
Sequence-Conflict 59
[Simon94, UniProt10a]
UniProt: (in Ref. 1; CAA63921);
Sequence-Conflict 230
[Simon94, UniProt10a]
UniProt: (in Ref. 1; CAA63921);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Baraquet06: Baraquet C, Theraulaz L, Guiral M, Lafitte D, Mejean V, Jourlin-Castelli C (2006). "TorT, a member of a new periplasmic binding protein family, triggers induction of the Tor respiratory system upon TMAO electron-acceptor binding in Escherichia coli." J Biol Chem 281(50):38189-99. PMID: 17040909

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Jourlin96: Jourlin C, Simon G, Pommier J, Chippaux M, Mejean V (1996). "The periplasmic TorT protein is required for trimethylamine N-oxide reductase gene induction in Escherichia coli." J Bacteriol 1996;178(4);1219-23. PMID: 8576063

Jourlin96a: Jourlin C, Bengrine A, Chippaux M, Mejean V (1996). "An unorthodox sensor protein (TorS) mediates the induction of the tor structural genes in response to trimethylamine N-oxide in Escherichia coli." Mol Microbiol 1996;20(6);1297-306. PMID: 8809780

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Simon94: Simon G, Mejean V, Jourlin C, Chippaux M, Pascal MC (1994). "The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes." J Bacteriol 1994;176(18);5601-6. PMID: 8083154

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, BIOCYC14A.