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Escherichia coli K-12 substr. MG1655 Polypeptide: putative curli production protein



Gene: csgC Accession Numbers: G6548 (EcoCyc), b1043, ECK1029

Synonyms: ycdE

Regulation Summary Diagram: ?

Summary:
An insertion in csgC does not affect production of curli, but abolishes the autoagglutinating ability which curliated cells normally possess [Hammar95].

Gene Citations: [Neidhardt96, Arnqvist94]

Locations: periplasmic space

Map Position: [1,104,184 -> 1,104,516] (23.8 centisomes)
Length: 333 bp / 110 aa

Molecular Weight of Polypeptide: 11.967 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003542 , EchoBASE:EB3190 , EcoGene:EG13414 , EcoliWiki:b1043 , OU-Microarray:b1043 , PortEco:csgC , Protein Model Portal:P52107 , RefSeq:NP_415561 , RegulonDB:G6548 , SMR:P52107 , String:511145.b1043 , UniProt:P52107

Relationship Links: InterPro:IN-FAMILY:IPR014491 , Pfam:IN-FAMILY:PF10610

Genetic Regulation Schematic: ?

GO Terms:

Cellular Component: GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: cell structure

Essentiality data for csgC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 8
[UniProt10]
UniProt: Non-Experimental Qualifier: potential;
Chain 9 -> 110
[UniProt10]
UniProt: Curli assembly protein csgC;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Arnqvist94: Arnqvist A, Olsen A, Normark S (1994). "Sigma S-dependent growth-phase induction of the csgBA promoter in Escherichia coli can be achieved in vivo by sigma 70 in the absence of the nucleoid-associated protein H-NS." Mol Microbiol 1994;13(6);1021-32. PMID: 7854117

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Hammar95: Hammar M, Arnqvist A, Bian Z, Olsen A, Normark S (1995). "Expression of two csg operons is required for production of fibronectin- and congo red-binding curli polymers in Escherichia coli K-12." Mol Microbiol 1995;18(4);661-70. PMID: 8817489

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Barnhart06: Barnhart MM, Lynem J, Chapman MR (2006). "GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli." J Bacteriol 188(14);5212-9. PMID: 16816193

Bougdour04: Bougdour A, Lelong C, Geiselmann J (2004). "Crl, a low temperature-induced protein in Escherichia coli that binds directly to the stationary phase sigma subunit of RNA polymerase." J Biol Chem 279(19);19540-50. PMID: 14978043

Brombacher03: Brombacher E, Dorel C, Zehnder AJ, Landini P (2003). "The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli." Microbiology 149(Pt 10);2847-57. PMID: 14523117

De02: De Wulf P, McGuire AM, Liu X, Lin EC (2002). "Genome-wide profiling of promoter recognition by the two-component response regulator CpxR-P in Escherichia coli." J Biol Chem 277(29);26652-61. PMID: 11953442

Fink12: Fink RC, Black EP, Hou Z, Sugawara M, Sadowsky MJ, Diez-Gonzalez F (2012). "Transcriptional responses of Escherichia coli K-12 and O157:H7 associated with lettuce leaves." Appl Environ Microbiol 78(6);1752-64. PMID: 22247152

Jubelin05: Jubelin G, Vianney A, Beloin C, Ghigo JM, Lazzaroni JC, Lejeune P, Dorel C (2005). "CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli." J Bacteriol 187(6);2038-49. PMID: 15743952

Ogasawara11: Ogasawara H, Yamamoto K, Ishihama A (2011). "Role of the Biofilm Master Regulator CsgD in Cross-Regulation between Biofilm Formation and Flagellar Synthesis." J Bacteriol 193(10);2587-97. PMID: 21421764

Pesavento08: Pesavento C, Becker G, Sommerfeldt N, Possling A, Tschowri N, Mehlis A, Hengge R (2008). "Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli." Genes Dev 22(17);2434-46. PMID: 18765794

PrigentCombaret01: Prigent-Combaret C, Brombacher E, Vidal O, Ambert A, Lejeune P, Landini P, Dorel C (2001). "Complex regulatory network controls initial adhesion and biofilm formation in Escherichia coli via regulation of the csgD gene." J Bacteriol 2001;183(24);7213-23. PMID: 11717281

Reshamwala11: Reshamwala SM, Noronha SB (2011). "Biofilm formation in Escherichia coli cra mutants is impaired due to down-regulation of curli biosynthesis." Arch Microbiol 193(10);711-22. PMID: 21559929

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.