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Escherichia coli K-12 substr. MG1655 Polypeptide: putative adhesion and penetration protein

Gene: ycgV Accession Numbers: G6629 (EcoCyc), b1202, ECK1190

Regulation Summary Diagram: ?

Regulation summary diagram for ycgV

YcgV is a potential adhesin. It was identified as a protein with similarity to antigen 43 (Ag43), a self-recognizing surface adhesin and member of the Autotransporter (AT) family of proteins [Zhai02, Roux05]. Deletion of the ycgV gene has no significant effect on adhesion or biofilm formation, while increased expression of YcgV causes an increase in adhesion to solid surfaces and increased biofilm formation [Roux05].

Citations: [ParadisBleau14]

Locations: membrane

Map Position: [1,252,308 <- 1,255,175] (26.99 centisomes, 97°)
Length: 2868 bp / 955 aa

Molecular Weight of Polypeptide: 99.685 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004035 , EchoBASE:EB3662 , EcoGene:EG13903 , EcoliWiki:b1202 , OU-Microarray:b1202 , PortEco:ycgV , Pride:P76017 , Protein Model Portal:P76017 , RefSeq:NP_415720 , RegulonDB:G6629 , SMR:P76017 , String:511145.b1202 , UniProt:P76017

Relationship Links: InterPro:IN-FAMILY:IPR003991 , InterPro:IN-FAMILY:IPR004899 , InterPro:IN-FAMILY:IPR005546 , InterPro:IN-FAMILY:IPR006315 , InterPro:IN-FAMILY:IPR011050 , InterPro:IN-FAMILY:IPR012332 , Pfam:IN-FAMILY:PF03212 , Pfam:IN-FAMILY:PF03797 , Prints:IN-FAMILY:PR01484 , Prosite:IN-FAMILY:PS51208 , Smart:IN-FAMILY:SM00869

In Paralogous Gene Group: 300 (3 members)

GO Terms:

Biological Process: GO:0043708 - cell adhesion involved in biofilm formation Inferred from experiment [Roux05]
GO:0007155 - cell adhesion Inferred by computational analysis [GOA01]
Cellular Component: GO:0019867 - outer membrane Inferred by computational analysis [GOA01]


Essentiality data for ycgV knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of putative adhesion and penetration protein with features indicated

Feature Class Location Citations Comment
Conserved-Region 687 -> 955
UniProt: Autotransporter;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

ParadisBleau14: Paradis-Bleau C, Kritikos G, Orlova K, Typas A, Bernhardt TG (2014). "A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism." PLoS Genet 10(1);e1004056. PMID: 24391520

Roux05: Roux A, Beloin C, Ghigo JM (2005). "Combined inactivation and expression strategy to study gene function under physiological conditions: application to identification of new Escherichia coli adhesins." J Bacteriol 187(3);1001-13. PMID: 15659678

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

Zhai02: Zhai Y, Saier MH (2002). "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes." Protein Sci 11(9);2196-207. PMID: 12192075

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Sep 3, 2015, biocyc13.