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Escherichia coli K-12 substr. MG1655 Polypeptide: membrane-associated protein



Gene: uidC Accession Numbers: G6866 (EcoCyc), b1615, ECK1610

Synonyms: gusC

Regulation Summary Diagram: ?

Summary:
The uidC gene is part of the uidABC operon. The gene products of uidB and uidC have been shown through expression studies and transport assays in a natural isolate of Escherichia coli, strain CE1, to be involved in secondary active transport of beta-glucuronides into the cell energized by the respiration-generated proton motive force. Further metabolism of the beta-glucuronides is performed by UidA. Membrane separation experiments find the UidC protein associated with the outer membrane. Expression of UidC alone from strain CE1 in strain KW1 (uid operon deletion) does not confer transport activity, but improves efficiency of transport by UidB by an unknown mechanism when the two are expressed simultaneously [Liang05].

Gene Citations: [Blanco82, Blattner97]

Locations: outer membrane

Map Position: [1,689,610 <- 1,690,875] (36.42 centisomes)
Length: 1266 bp / 421 aa

Molecular Weight of Polypeptide: 46.648 kD (from nucleotide sequence), 44 kD (experimental) [Liang05 ]

Unification Links: ASAP:ABE-0005405 , DIP:DIP-11088N , EchoBASE:EB2534 , EcoGene:EG12668 , EcoliWiki:b1615 , Mint:MINT-1299860 , OU-Microarray:b1615 , PortEco:uidC , PR:PRO_000024173 , Protein Model Portal:Q47706 , RefSeq:NP_416132 , RegulonDB:G6866 , String:511145.b1615 , UniProt:Q47706

Relationship Links: InterPro:IN-FAMILY:IPR005318 , InterPro:IN-FAMILY:IPR023614 , Pfam:IN-FAMILY:PF03573

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0055085 - transmembrane transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0015288 - porin activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0009279 - cell outer membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Liang05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046930 - pore complex Inferred by computational analysis [UniProtGOA11a]

Essentiality data for uidC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 23
[Liang05, UniProt11]
.
Chain 24 -> 421
[UniProt09]
UniProt: Membrane-associated protein uidC;
Sequence-Conflict 106 -> 109
[Liang94, UniProt10]
Alternate sequence: GFAL → ALP; UniProt: (in Ref. 1; AAA68925);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Blanco82: Blanco C, Ritzenthaler P, Mata-Gilsinger M (1982). "Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene." J Bacteriol 149(2);587-94. PMID: 6276362

Blattner97: Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997). "The complete genome sequence of Escherichia coli K-12." Science 277(5331);1453-74. PMID: 9278503

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Liang05: Liang WJ, Wilson KJ, Xie H, Knol J, Suzuki S, Rutherford NG, Henderson PJ, Jefferson RA (2005). "The gusBC genes of Escherichia coli encode a glucuronide transport system." J Bacteriol 187(7);2377-85. PMID: 15774881

Liang94: Liang W.J., Wilson K.J., Jefferson R.A. (1994). "The gus genes of Escherichia coli." Data submission to EMBL/GenBank/DDBJ databases on 1994-10.

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Blanco85: Blanco C, Mata-Gilsinger M, Ritzenthaler P (1985). "The use of gene fusions to study the expression of uidR, a negative regulatory gene of Escherichia coli K-12." Gene 36(1-2);159-67. PMID: 3934044

Blanco86a: Blanco C, Ritzenthaler P, Mata-Gilsinger M (1986). "Negative dominant mutations of the uidR gene in Escherichia coli: genetic proof for a cooperative regulation of uidA expression." Genetics 112(2);173-82. PMID: 3079718

Blanco87: Blanco C (1987). "Transcriptional and translational signals of the uidA gene in Escherichia coli K12." Mol Gen Genet 208(3);490-8. PMID: 2823062

Rodionov00: Rodionov DA, Mironov AA, Rakhmaninova AB, Gelfand MS (2000). "Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria." Mol Microbiol 38(4);673-83. PMID: 11115104


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13A.