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Escherichia coli K-12 substr. MG1655 Transporter: N-acetylmuramic acid PTS permease / anhydro-N-acetylmuramic acid permease



Gene: murP Accession Numbers: G7264 (EcoCyc), b2429, ECK2424

Synonyms: yfeV, EIIBCNAcMur, Enzyme IIBCNAcMur

Regulation Summary Diagram: ?

Component of: N-acetylmuramic acid PTS permease (extended summary available)

Summary:
murP encodes the permease component of the N-acetylmuramic acid PTS transport system. MurP contains PTS Enzyme IIB and IIC domains [Dahl04].

MurP is required for the uptake of anhydrous N-acetylmuramic (anhMurNAc) acid from the medium. MurP transports but does not phosphorylate anhMurNAc. The anmK encoded anhydro-N-acetylmuramic acid kinase is required to convert imported anhMurNAc to MurNAc-P. E. coli K-12 cannot use anhMurNAc as the sole source of carbon [Uehara05, Uehara06].

Gene Citations: [Jaeger08, Maciag11]

Locations: inner membrane

Map Position: [2,544,695 -> 2,546,119] (54.85 centisomes)
Length: 1425 bp / 474 aa

Molecular Weight of Polypeptide: 49.802 kD (from nucleotide sequence), 37 kD (experimental) [Dahl04 ]

Unification Links: ASAP:ABE-0008009 , EchoBASE:EB3915 , EcoGene:EG14163 , EcoliWiki:b2429 , ModBase:P77272 , OU-Microarray:b2429 , PortEco:murP , PR:PRO_000023320 , Pride:P77272 , Protein Model Portal:P77272 , RefSeq:NP_416924 , RegulonDB:G7264 , SMR:P77272 , String:511145.b2429 , UniProt:P77272

Relationship Links: InterPro:IN-FAMILY:IPR001996 , InterPro:IN-FAMILY:IPR003352 , InterPro:IN-FAMILY:IPR013013 , InterPro:IN-FAMILY:IPR018113 , Pfam:IN-FAMILY:PF00367 , Pfam:IN-FAMILY:PF02378 , Prosite:IN-FAMILY:PS01035 , Prosite:IN-FAMILY:PS51098 , Prosite:IN-FAMILY:PS51103

In Paralogous Gene Group: 417 (2 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Dahl04]
GO:0034219 - carbohydrate transmembrane transport Inferred from experiment [Dahl04]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0090588 - protein-phosphocysteine-N-acetylmuramate phosphotransferase system transporter activity Inferred by computational analysis Inferred from experiment [Dahl04]
GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Dahl04]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Dahl04]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for murP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 27-Apr-2014 by Mackie A , Macquarie University


Enzymatic reaction of: anhydro-N-acetylmuramic acid permease


Subunit of: N-acetylmuramic acid PTS permease

Synonyms: Enzyme IINAcMur, EIINAcMur

Subunit composition of N-acetylmuramic acid PTS permease = [MurP][Crr]
         N-acetylmuramic acid PTS permease / anhydro-N-acetylmuramic acid permease = MurP (summary available)
         Enzyme IIAGlc; Crr = Crr (extended summary available)

Summary:
MurP, the N-acetylmuramic acid PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed in [Postma93].

murP encodes a protein containing Enyme IIB (EIIB) and Enzyme IIC (EIIC) domains. Strains lacking murP are unable to grown on N-acetylmuramic acid as the sole source of carbon. Strains lacking crr, which encodes an Enzyme IIA (EIIA) protein are also unable to grow on N-acetylmuramic acid indicating that MurP, which lacks an EIIA domain requires Crr for activity. Sequence analysis suggests that a conserved cysteine residue at position 29 is the site of phosphotransfer [Dahl04]. MurP/Crr is a member of the PTS Glucose-Glucoside family of transporters [Saier14]. The product of MurP transport, N-acetyl-β-muramate 6-phosphate, is further degraded by the action of the MurQ etherase.

The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTSsugar, PtsI (Enzyme I) and PtsH (HPr), as well as the Crr protein and the two domains of the MurP protein can be represented as:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIAglc-Phis90 → Enzyme IIB-Pcys29 - (Enzyme IIC) → N-acetylmuramic acid-6-P.

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis Inferred from experiment [Dahl04]
Molecular Function: GO:0090588 - protein-phosphocysteine-N-acetylmuramate phosphotransferase system transporter activity Inferred from experiment Inferred by computational analysis [Dahl04]

Credits:
Last-Curated ? 16-Apr-2014 by Mackie A , Macquarie University


Enzymatic reaction of: N-acetylmuramic acid PTS permease

Synonyms: Transport of N-acetylmuramate

pH(opt): 6 [BRENDA14, Saier80], 6.5 [BRENDA14, Jacobson83], 7 [BRENDA14, Jacobson79], 7.5 [BRENDA14, Sutrina87], 8.5 [BRENDA14, Jacobson83], 9 [BRENDA14, Jacobson79], 9.5 [BRENDA14, Saier80]


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 89
[UniProt09]
UniProt: PTS EIIB type-1;
Active-Site 29
[UniProt10a]
UniProt: Phosphocysteine intermediate; for EIIB activity; Non-Experimental Qualifier: by similarity;
Conserved-Region 115 -> 474
[UniProt09]
UniProt: PTS EIIC type-1;
Transmembrane-Region 124 -> 144
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 158 -> 178
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 181 -> 201
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 218 -> 238
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 261 -> 281
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 302 -> 322
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 335 -> 355
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 369 -> 389
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 394 -> 414
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 441 -> 461
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Dahl04: Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C (2004). "Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid." J Bacteriol 186(8);2385-92. PMID: 15060041

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jacobson79: Jacobson GR, Lee CA, Saier MH (1979). "Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system." J Biol Chem 254(2);249-52. PMID: 368051

Jacobson83: Jacobson GR, Lee CA, Leonard JE, Saier MH (1983). "Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease." J Biol Chem 258(17);10748-56. PMID: 6350293

Jaeger08: Jaeger T, Mayer C (2008). "The transcriptional factors MurR and catabolite activator protein regulate N-acetylmuramic acid catabolism in Escherichia coli." J Bacteriol 190(20);6598-608. PMID: 18723630

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Maciag11: Maciag A, Peano C, Pietrelli A, Egli T, De Bellis G, Landini P (2011). "In vitro transcription profiling of the {sigma}S subunit of bacterial RNA polymerase: re-definition of the {sigma}S regulon and identification of {sigma}S-specific promoter sequence elements." Nucleic Acids Res 39(13);5338-55. PMID: 21398637

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

Saier80: Saier MH (1980). "Catalytic activities associated with the enzymes II of the bacterial phosphotransferase system." J Supramol Struct 14(3);281-94. PMID: 7012451

Sutrina87: Sutrina SL, Waygood EB, Grenier FC, Saier MH (1987). "HPr/HPr-P phosphoryl exchange reaction catalyzed by the mannitol specific enzyme II of the bacterial phosphotransferase system." J Biol Chem 262(6);2636-41. PMID: 3102473

Uehara05: Uehara T, Suefuji K, Valbuena N, Meehan B, Donegan M, Park JT (2005). "Recycling of the anhydro-N-acetylmuramic acid derived from cell wall murein involves a two-step conversion to N-acetylglucosamine-phosphate." J Bacteriol 187(11);3643-9. PMID: 15901686

Uehara06: Uehara T, Suefuji K, Jaeger T, Mayer C, Park JT (2006). "MurQ Etherase is required by Escherichia coli in order to metabolize anhydro-N-acetylmuramic acid obtained either from the environment or from its own cell wall." J Bacteriol 188(4);1660-2. PMID: 16452451

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc13.