Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Enzyme: phosphate acetyltransferase



Gene: eutD Accession Numbers: G7288 (EcoCyc), b2458, ECK2453

Synonyms: ypfA, eutI

Regulation Summary Diagram: ?

Subunit composition of phosphate acetyltransferase = [EutD]2
         phosphate acetyltransferase monomer = EutD

Summary:
The EutD protein has phosphotransacetylase activity. Expression of EutD - either from a plasmid or by inducing expression of the eut operon with ethanolamine and vitamin B12 - can rescue the growth defect of a pta acs mutant for growth on acetate as the sole source of carbon [Bologna10].

eutD shows differential codon adaptation, resulting in differential translation efficiency signatures, in thermophilic microbes. It was therefore predicted to play a role in the heat shock response. A eutD deletion mutant was shown to be more sensitive than wild-type specifically to heat shock, but not other stresses [Krisko14].

Citations: [Wang14b]

Locations: cytosol

Map Position: [2,570,511 <- 2,571,527] (55.4 centisomes)
Length: 1017 bp / 338 aa

Molecular Weight of Polypeptide: 36.067 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008097 , EchoBASE:EB3940 , EcoGene:EG14188 , EcoliWiki:b2458 , ModBase:P77218 , OU-Microarray:b2458 , PortEco:eutD , PR:PRO_000022538 , Protein Model Portal:P77218 , RefSeq:NP_416953 , RegulonDB:G7288 , SMR:P77218 , String:511145.b2458 , UniProt:P77218

Relationship Links: InterPro:IN-FAMILY:IPR002505 , InterPro:IN-FAMILY:IPR004614 , InterPro:IN-FAMILY:IPR012147 , PDB:Structure:1VMI , Pfam:IN-FAMILY:PF01515

In Paralogous Gene Group: 297 (4 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009408 - response to heat Inferred from experiment [Krisko14]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0046336 - ethanolamine catabolic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0008959 - phosphate acetyltransferase activity Inferred from experiment [Bologna10]
GO:0042803 - protein homodimerization activity Inferred from experiment [Bologna10]
GO:0016407 - acetyltransferase activity Inferred by computational analysis [GOA01]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization

Essentiality data for eutD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Created 01-Dec-2010 by Keseler I , SRI International
Last-Curated ? 15-May-2014 by Keseler I , SRI International


Enzymatic reaction of: phosphate acetyltransferase

Synonyms: phosphotransacetylase

EC Number: 2.3.1.8

acetyl-CoA + phosphate <=> acetyl phosphate + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Bologna10]

In Pathways: superpathway of acetate utilization and formation , acetate formation from acetyl-CoA I , mixed acid fermentation

Summary:
The acetyl-phosphate forming reaction is catalyzed at nearly 4-times lower rates that the acetyl-CoA forming reaction; however, differences in Km result in similar catalytic efficiency in both directions [Bologna10].

Activators (Unknown Mechanism): pyruvate [Bologna10]

Kinetic Parameters:

Substrate
Km (μM)
Citations
acetyl-CoA
9.5
[Bologna10]
coenzyme A
32.7
[Bologna10]
acetyl phosphate
311.7
[Bologna10]

pH(opt): 7.8 [Bologna10]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bologna10: Bologna FP, Campos-Bermudez VA, Saavedra DD, Andreo CS, Drincovich MF (2010). "Characterization of Escherichia coli EutD: a phosphotransacetylase of the ethanolamine operon." J Microbiol 48(5);629-36. PMID: 21046341

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Krisko14: Krisko A, Copi T, Gabaldon T, Lehner B, Supek F (2014). "Inferring gene function from evolutionary change in signatures of translation efficiency." Genome Biol 15(3);R44. PMID: 24580753

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Wang14b: Wang JF, Meng HL, Xiong ZQ, Zhang SL, Wang Y (2014). "Identification of novel knockout and up-regulated targets for improving isoprenoid production in E. coli." Biotechnol Lett. PMID: 24658737

Other References Related to Gene Regulation

Oberto10: Oberto J (2010). "FITBAR: a web tool for the robust prediction of prokaryotic regulons." BMC Bioinformatics 11;554. PMID: 21070640


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC13B.