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Escherichia coli K-12 substr. MG1655 Enzyme: protein-tyrosine-phosphatase / phosphoprotein phosphatase



Gene: pphB Accession Numbers: G7415 (EcoCyc), b2734, ECK2729

Synonyms: ygbH, prpB, protein phosphatase 2

Regulation Summary Diagram: ?

Summary:
PrpA and PrpB are phosphatases that act in transduction of the misfolded protein stress signal in a pathway that includes the CpxR-CpxA two-component system and which activates transcription of the htrA gene [Missiakas97]. PrpA and PrpB exhibit phosphatase activity toward phosphorylated serine/threonine and phosphorylated tyrosine in protein substrates [Missiakas97].

PrpA and PrpB have similarity to the phosphoprotein phosphatase of bacteriophage lambda (lambdaPP) [White01] and to Salmonella enterica serovar Typhimurium PrpA and PrpB proteins [Shi01].

Reviews: [Pallen97, Shi04].

Map Position: [2,857,782 -> 2,858,438] (61.59 centisomes)
Length: 657 bp / 218 aa

Molecular Weight of Polypeptide: 25.098 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008980 , EchoBASE:EB2905 , EcoGene:EG13102 , EcoliWiki:b2734 , ModBase:P55799 , OU-Microarray:b2734 , PortEco:pphB , PR:PRO_000023578 , Protein Model Portal:P55799 , RefSeq:NP_417214 , RegulonDB:G7415 , SMR:P55799 , String:511145.b2734 , Swiss-Model:P55799 , UniProt:P55799

Relationship Links: InterPro:IN-FAMILY:IPR004843 , InterPro:IN-FAMILY:IPR006186 , Pfam:IN-FAMILY:PF00149 , Prosite:IN-FAMILY:PS00125

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0016311 - dephosphorylation Inferred by computational analysis Inferred from experiment [Missiakas97, UniProtGOA11a, GOA01]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0004721 - phosphoprotein phosphatase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01, Missiakas97]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes adaptations other (mechanical, nutritional, oxidative stress)
information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation posttranscriptional inhibition / activation of enzymes

Essentiality data for pphB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Enzymatic reaction of: protein-tyrosine-phosphatase

Synonyms: phosphotyrosine phosphatase, protein-tyrosine-phosphate phosphohydrolase

EC Number: 3.1.3.48

a [protein]-L-tyrosine phosphate + H2O <=> a [protein]-L-tyrosine + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Citations: [Missiakas97]


Enzymatic reaction of: phosphoprotein phosphatase

Synonyms: phosphoprotein phosphohydrolase, serine/threonine specific protein phosphatase

EC Number: 3.1.3.16

a [protein] (L-serine/L-threonine) phosphate + H2O <=> a [protein]-(L-serine/L-threonine) + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Citations: [Missiakas97]


Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 22
[UniProt10]
UniProt: Iron; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 24
[UniProt10]
UniProt: Iron; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 51
[UniProt10]
UniProt: Iron; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 77
[UniProt10]
UniProt: Manganese; Non-Experimental Qualifier: by similarity;
Mutagenesis-Variant 78
[Missiakas97, UniProt11]
Alternate sequence: H → N; UniProt: In prpB17; loss of function.
Active-Site 78
[UniProt10]
UniProt: Proton donor; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 187
[UniProt10]
UniProt: Manganese; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Missiakas97: Missiakas D, Raina S (1997). "Signal transduction pathways in response to protein misfolding in the extracytoplasmic compartments of E. coli: role of two new phosphoprotein phosphatases PrpA and PrpB." EMBO J 16(7);1670-85. PMID: 9130712

Pallen97: Pallen MJ, Wren BW (1997). "The HtrA family of serine proteases." Mol Microbiol 26(2);209-21. PMID: 9383148

Shi01: Shi L, Kehres DG, Maguire ME (2001). "The PPP-family protein phosphatases PrpA and PrpB of Salmonella enterica serovar Typhimurium possess distinct biochemical properties." J Bacteriol 183(24);7053-7. PMID: 11717262

Shi04: Shi L (2004). "Manganese-dependent protein O-phosphatases in prokaryotes and their biological functions." Front Biosci 9;1382-97. PMID: 14977554

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

White01: White DJ, Reiter NJ, Sikkink RA, Yu L, Rusnak F (2001). "Identification of the high affinity Mn2+ binding site of bacteriophage lambda phosphoprotein phosphatase: effects of metal ligand mutations on electron paramagnetic resonance spectra and phosphatase activities." Biochemistry 40(30);8918-29. PMID: 11467953


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc12.