Escherichia coli K-12 substr. MG1655 Polypeptide: putative NAD(P)-linked reductase that acts in starvation-associated mutation

Gene: tas Accession Numbers: G7462 (EcoCyc), b2834, ECK2830

Synonyms: ygdS

Regulation Summary Diagram: ?

Regulation summary diagram for tas

Tas is required for starvation-associated mutational suppression of a tyrA14(oc) allele [Timms98]. Tas has similarity to aldo-keto reductases [Timms98].

A tas mutant does not exhibit starvation-associated mutation [Timms98]. Multicopy tas expression suppresses the tyrosine auxotrophy of a tyrA14(oc) leu308(am) mutant, which has a prephenate dehydrogenase defect [Timms98].

Strain differences have been described [Johnson01].

Review: [Lapthorn13]

Locations: cytosol

Map Position: [2,969,619 -> 2,970,659] (64.0 centisomes, 230°)
Length: 1041 bp / 346 aa

Molecular Weight of Polypeptide: 38.5 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009298 , DIP:DIP-48107N , EchoBASE:EB2898 , EcoGene:EG13093 , EcoliWiki:b2834 , ModBase:P0A9T4 , OU-Microarray:b2834 , PortEco:tas , PR:PRO_000024027 , Pride:P0A9T4 , Protein Model Portal:P0A9T4 , RefSeq:NP_417311 , RegulonDB:G7462 , SMR:P0A9T4 , String:511145.b2834 , UniProt:P0A9T4

Relationship Links: InterPro:IN-FAMILY:IPR001395 , InterPro:IN-FAMILY:IPR020471 , InterPro:IN-FAMILY:IPR023210 , Panther:IN-FAMILY:PTHR11732 , PDB:Structure:1LQA , Pfam:IN-FAMILY:PF00248 , Prints:IN-FAMILY:PR00069

In Paralogous Gene Group: 65 (9 members)

GO Terms:

Biological Process: GO:0034198 - cellular response to amino acid starvation Inferred from experiment [Timms98]
GO:0006259 - DNA metabolic process
GO:0042594 - response to starvation
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0004033 - aldo-keto reductase (NADP) activity Author statement [Obmolova03]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08]

MultiFun Terms: Genes ORFs
MultiFun cell processes adaptations starvation
MultiFun information transfer DNA related
MultiFun metabolism

Essentiality data for tas knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of putative NAD(P)-linked reductase that acts in starvation-associated mutation with features indicated

Feature Class Location Attached Group Citations Comment
Active-Site 53  
UniProt: Proton donor.
Nucleotide-Phosphate-Binding-Region 234 -> 244 NADP+
[Obmolova03, UniProt15]
UniProt: NADP.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Johnson01: Johnson JM, Ding W, Henkhaus J, Fix D (2001). "Identification of a mutation causing increased expression of the tas gene in Escherichia coli FX-11." Mutat Res 479(1-2);121-30. PMID: 11470487

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lapthorn13: Lapthorn AJ, Zhu X, Ellis EM (2013). "The diversity of microbial aldo/keto reductases from Escherichia coli K12." Chem Biol Interact 202(1-3);168-77. PMID: 23103600

Obmolova03: Obmolova G, Teplyakov A, Khil PP, Howard AJ, Camerini-Otero RD, Gilliland GL (2003). "Crystal structure of the Escherichia coli Tas protein, an NADP(H)-dependent aldo-keto reductase." Proteins 53(2);323-5. PMID: 14517983

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Timms98: Timms AR, Bridges BA (1998). "Reversion of the tyrosine ochre strain Escherichia coli WU3610 under starvation conditions depends on a new gene tas." Genetics 148(4);1627-35. PMID: 9560382

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sat Oct 10, 2015, biocyc13.