Escherichia coli K-12 substr. MG1655 Enzyme: propionyl-CoA:succinate CoA transferase

Gene: scpC Accession Numbers: G7517 (EcoCyc), b2920, ECK2916

Synonyms: ygfH

Regulation Summary Diagram: ?

Regulation summary diagram for scpC

The scpC-encoded enzyme is a CoA transferase. This reaction is suggested to be part of a pathway of succinate decarboxylation whose metabolic function is unknown [Haller00].

Deletion of scpC increases heterologous production of 6-deoxyerythronolide B (6dEB), the macrolactone precursor of erythromycin [Zhang10, Boghigian11].

Locations: cytosol

Map Position: [3,062,824 -> 3,064,302] (66.01 centisomes, 238°)
Length: 1479 bp / 492 aa

Molecular Weight of Polypeptide: 53.824 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009583 , DIP:DIP-12167N , EchoBASE:EB2800 , EcoGene:EG12973 , EcoliWiki:b2920 , Mint:MINT-1232787 , ModBase:P52043 , OU-Microarray:b2920 , PortEco:scpC , Pride:P52043 , Protein Model Portal:P52043 , RefSeq:NP_417395 , RegulonDB:G7517 , SMR:P52043 , String:511145.b2920 , Swiss-Model:P52043 , UniProt:P52043

Relationship Links: InterPro:IN-FAMILY:IPR003702 , InterPro:IN-FAMILY:IPR017821 , InterPro:IN-FAMILY:IPR026888 , Panther:IN-FAMILY:PTHR21432:SF10 , Pfam:IN-FAMILY:PF02550 , Pfam:IN-FAMILY:PF13336

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for scpC

GO Terms:

Biological Process: GO:0006084 - acetyl-CoA metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0043821 - propionyl-CoA:succinate CoA-transferase activity Inferred from experiment [Haller00]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism central intermediary metabolism unassigned reversible reactions

Essentiality data for scpC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Last-Curated ? 13-Sep-2011 by Keseler I , SRI International

Enzymatic reaction of: propionyl-CoA:succinate CoA transferase

EC Number: 2.8.3.-

propanoyl-CoA + succinate <=> propanoate + succinyl-CoA

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Haller00]

Alternative Substrates for propanoate [Comment 5 ]: acetate [Haller00 ] , butanoate [Haller00 ]

In Pathways: conversion of succinate to propanoate

Succinyl-CoA and propionyl-CoA are the preferred CoA donors, and succinate and propionate are the preferred acceptors. Acetate and butyrate are less efficient as CoA acceptors [Haller00].

Kinetic Parameters:

Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)

Sequence Features

Protein sequence of propionyl-CoA:succinate CoA transferase with features indicated

Feature Class Location Citations Comment
Protein-Segment 260 -> 264
UniProt: Coenzyme A binding; Sequence Annotation Type: region of interest.
Active-Site 286
UniProt: 5-glutamyl coenzyme A thioester intermediate.
Amino-Acid-Sites-That-Bind 376
UniProt: Coenzyme A.
Amino-Acid-Sites-That-Bind 380
UniProt: Coenzyme A; via amide nitrogen.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Boghigian11: Boghigian BA, Zhang H, Pfeifer BA (2011). "Multi-factorial engineering of heterologous polyketide production in Escherichia coli reveals complex pathway interactions." Biotechnol Bioeng 108(6);1360-71. PMID: 21337322

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Haller00: Haller T, Buckel T, Retey J, Gerlt JA (2000). "Discovering new enzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli." Biochemistry 2000;39(16);4622-9. PMID: 10769117

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt14a: UniProt Consortium (2014). "UniProt version 2014-08 released on 2014-08-01 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang10: Zhang H, Boghigian BA, Pfeifer BA (2010). "Investigating the role of native propionyl-CoA and methylmalonyl-CoA metabolism on heterologous polyketide production in Escherichia coli." Biotechnol Bioeng 105(3);567-73. PMID: 19806677

Other References Related to Gene Regulation

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, BIOCYC14B.