Escherichia coli K-12 substr. MG1655 Polypeptide: QseB transcriptional activator

Gene: qseB Accession Numbers: G7575 (EcoCyc), b3025, ECK3016

Synonyms: ygiX, QseB response regulator

Regulation Summary Diagram: ?

Regulation summary diagram for qseB

Alternative forms of QseB transcriptional activator: QseB-Phosphorylated DNA-binding transcriptional activator (extended summary available)

Gene Citations: [Sperandio02, Clarke05]

Locations: cytosol

Map Position: [3,167,850 -> 3,168,509] (68.28 centisomes, 246°)
Length: 660 bp / 219 aa

Molecular Weight of Polypeptide: 24.678 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009936 , EchoBASE:EB2844 , EcoGene:EG13026 , EcoliWiki:b3025 , ModBase:P52076 , OU-Microarray:b3025 , PortEco:qseB , PR:PRO_000023666 , Protein Model Portal:P52076 , RefSeq:NP_417497 , RegulonDB:G7575 , SMR:P52076 , String:511145.b3025 , UniProt:P52076

Relationship Links: InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR001867 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR011991 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF00486 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00448 , Smart:IN-FAMILY:SM00862

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

QseBC Two-Component Signal Transduction System, quorum sensing related :
QseB + QseC sensory histidine kinase - phosphorylated → QseB-Pasp51 + QseC

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for qseB

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11, Sperandio02]
GO:0000160 - phosphorelay signal transduction system Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
regulation type of regulation transcriptional level activator
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)
regulation type of regulation transcriptional level

DNA binding site length: 13 base-pairs

Symmetry: Inverted Repeat

Transcription Units regulated by related protein QseB-Phosphorylated DNA-binding transcriptional activator (2 total):

Transcription-unit diagram

Transcription-unit diagram

Essentiality data for qseB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 28-Dec-2005 by Keseler I , SRI International

Sequence Features

Protein sequence of QseB transcriptional activator with features indicated

Feature Class Location Citations Comment State
Conserved-Region 1 -> 116
UniProt: Response regulatory;
4-aspartylphosphate-Modification 51
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Clarke05: Clarke MB, Sperandio V (2005). "Transcriptional autoregulation by quorum sensing Escherichia coli regulators B and C (QseBC) in enterohaemorrhagic E. coli (EHEC)." Mol Microbiol 58(2);441-55. PMID: 16194231

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Sperandio02: Sperandio V, Torres AG, Kaper JB (2002). "Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli." Mol Microbiol 43(3);809-21. PMID: 11929534

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Clarke05a: Clarke MB, Sperandio V (2005). "Transcriptional regulation of flhDC by QseBC and sigma (FliA) in enterohaemorrhagic Escherichia coli." Mol Microbiol 57(6);1734-49. PMID: 16135237

Guckes13: Guckes KR, Kostakioti M, Breland EJ, Gu AP, Shaffer CL, Martinez CR, Hultgren SJ, Hadjifrangiskou M (2013). "Strong cross-system interactions drive the activation of the QseB response regulator in the absence of its cognate sensor." Proc Natl Acad Sci U S A 110(41);16592-7. PMID: 24062463

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, BIOCYC14A.