Escherichia coli K-12 substr. MG1655 Polypeptide: protein required for KefB activity

Gene: kefG Accession Numbers: G7717 (EcoCyc), b3351, ECK3339

Synonyms: yheR

Regulation Summary Diagram: ?

Regulation summary diagram for kefG

KefG is required for KefB activity [Miller00a].

Based on sequence similarity, KefG is predicted to be an NAD(P)H dehydrogenase [Reed03]. However, the protein appears to lack conserved essential residues [Miller00a].

Locations: cytosol, inner membrane

Map Position: [3,478,629 <- 3,479,183] (74.98 centisomes, 270°)
Length: 555 bp / 184 aa

Molecular Weight of Polypeptide: 20.958 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010951 , EchoBASE:EB2739 , EcoGene:EG12902 , EcoliWiki:b3351 , ModBase:P0A756 , OU-Microarray:b3351 , PortEco:kefG , PR:PRO_000023064 , Pride:P0A756 , Protein Model Portal:P0A756 , RefSeq:NP_417810 , RegulonDB:G7717 , SMR:P0A756 , String:511145.b3351 , Swiss-Model:P0A756 , UniProt:P0A756

Relationship Links: InterPro:IN-FAMILY:IPR003680 , InterPro:IN-FAMILY:IPR023947 , InterPro:IN-FAMILY:IPR029039 , Pfam:IN-FAMILY:PF02525

In Paralogous Gene Group: 18 (3 members)

GO Terms:

Biological Process: GO:0006813 - potassium ion transport Inferred by computational analysis [GOA01a]
GO:0032414 - positive regulation of ion transmembrane transporter activity Inferred by computational analysis [GOA06]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a]
GO:1901381 - positive regulation of potassium ion transmembrane transport Inferred by computational analysis [GOA06]
Molecular Function: GO:0003955 - NAD(P)H dehydrogenase (quinone) activity Inferred by computational analysis [GOA06, GOA01]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: metabolism

Essentiality data for kefG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 21-Dec-2005 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Miller00a: Miller S, Ness LS, Wood CM, Fox BC, Booth IR (2000). "Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli." J Bacteriol 182(22);6536-40. PMID: 11053405

Reed03: Reed JL, Vo TD, Schilling CH, Palsson BO (2003). "An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)." Genome Biol 4(9);R54. PMID: 12952533

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, biocyc13.