Escherichia coli K-12 substr. MG1655 Polypeptide: IS150 conserved protein InsB

Gene: insK Accession Numbers: G7777 (EcoCyc), b3558, ECK3546

Synonyms: dinS, yi5B, t150

Regulation Summary Diagram: ?

Regulation summary diagram for insK

InsB is one of three proteins coded for by the IS150 insertion element. Its function is unknown.

The IS150 insertion element, which is found singly or in many copies in various E. coli isolates, codes for three proteins, InsA, InsB and InsAB [Schwartz88, Schwartz88a, Hall89a]. Although InsA is translated normally, both InsB and InsAB translation require -1 frameshifts. InsAB translation begins from the same start codon as InsA translation, but a -1 frameshift allows readthrough on the former InsA stop codon, yielding the much longer protein InsAB. InsB translation begins at an ATG within the InsA sequence, requiring a -1 frameshift to properly translate it. In vivo, InsB is generated at a much lower rate than InsA or InsAB. The frameshift that generates InsAB rather than InsA occurs about a third of the time. Disruption of a putative stem-loop region following the frameshift site reduces InsAB expression dramatically [Vogele91].

InsAB is the transposase for the IS150 insertion element. Though a lack of InsB has no effect on the process, loss of separate InsA leads to a 5-fold increase in transposition of IS150. However, this lack of independent InsA also leads to a dramatic increase in generation of free circular and linear IS150 elements, suggesting InsA plays a role in preventing abortive transposition events [Haas02].

A much smaller LexA-regulated gene designated dinS has been identified within the coding region for InsB [Fernandez00].
Note that although this page says "Exons/Introns" it actually describes alternative splicing.

Locations: cytosol

Map Position: [3,719,177 -> 3,720,072] (80.16 centisomes, 289°)
Length: 896 bp / 298 aa

Molecular Weight of Polypeptide: 35.165 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011622 , EchoBASE:EB4760 , EcoGene:EG40011 , EcoliWiki:b3558 , ModBase:P19769 , OU-Microarray:b3558 , PortEco:insK , Protein Model Portal:P19769 , RefSeq:NP_418015 , RegulonDB:G7777 , String:511145.b3558 , UniProt:P19769

Relationship Links: EcoO157Cyc:Homolog:Z1573 , EcoO157Cyc:Homolog:Z1573-MONOMER , EcoO157Cyc:Homolog:Z1957 , EcoO157Cyc:Homolog:Z1957-MONOMER , InterPro:IN-FAMILY:IPR001584 , InterPro:IN-FAMILY:IPR012337 , InterPro:IN-FAMILY:IPR025948 , Pfam:IN-FAMILY:PF00665 , Pfam:IN-FAMILY:PF13276 , Prosite:IN-FAMILY:PS50994

Genetic Regulation Schematic: ?

Genetic regulation schematic for insK

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Fernandez00]
GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11]
GO:0015074 - DNA integration Inferred by computational analysis [GOA01]
GO:0032196 - transposition Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: extrachromosomal transposon related

Essentiality data for insK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]

Last-Curated ? 05-May-2006 by Shearer A , SRI International

Sequence Features

Protein sequence of IS150 conserved protein InsB with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 213
[Schwartz88a, UniProt10a]
[Hall89a, UniProt10a]
Y → A: (in Ref. 1; CAA30086);
Y → E: (in Ref. 2; no nucleotide entry);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram



Schematic showing introns, exons and/or isoforms of insK

Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fernandez00: Fernandez De Henestrosa AR, Ogi T, Aoyagi S, Chafin D, Hayes JJ, Ohmori H, Woodgate R (2000). "Identification of additional genes belonging to the LexA regulon in Escherichia coli." Mol Microbiol 35(6);1560-72. PMID: 10760155

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Haas02: Haas M, Rak B (2002). "Escherichia coli insertion sequence IS150: transposition via circular and linear intermediates." J Bacteriol 184(21);5833-41. PMID: 12374815

Hall89a: Hall BG, Parker LL, Betts PW, DuBose RF, Sawyer SA, Hartl DL (1989). "IS103, a new insertion element in Escherichia coli: characterization and distribution in natural populations." Genetics 121(3);423-31. PMID: 2541046

Schwartz88: Schwartz E, Herberger C, Rak B (1988). "Second-element turn-on of gene expression in an IS1 insertion mutant." Mol Gen Genet 211(2);282-9. PMID: 2832704

Schwartz88a: Schwartz E, Kroger M, Rak B (1988). "IS150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element." Nucleic Acids Res 16(14B);6789-802. PMID: 2841644

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Vogele91: Vogele K, Schwartz E, Welz C, Schiltz E, Rak B (1991). "High-level ribosomal frameshifting directs the synthesis of IS150 gene products." Nucleic Acids Res 19(16);4377-85. PMID: 1653413

Other References Related to Gene Regulation

GamaCastro08: Gama-Castro S, Jimenez-Jacinto V, Peralta-Gil M, Santos-Zavaleta A, Penaloza-Spinola MI, Contreras-Moreira B, Segura-Salazar J, Muniz-Rascado L, Martinez-Flores I, Salgado H, Bonavides-Martinez C, Abreu-Goodger C, Rodriguez-Penagos C, Miranda-Rios J, Morett E, Merino E, Huerta AM, Trevino-Quintanilla L, Collado-Vides J (2008). "RegulonDB (version 6.0): gene regulation model of Escherichia coli K-12 beyond transcription, active (experimental) annotated promoters and Textpresso navigation." Nucleic Acids Res 36(Database issue);D120-4. PMID: 18158297

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, biocyc13.