Escherichia coli K-12 substr. MG1655 Polypeptide: DcuR transcriptional activator

Gene: dcuR Accession Numbers: G7826 (EcoCyc), b4124, ECK4117

Synonyms: yjdG, DcuR response regulator

Regulation Summary Diagram: ?

Regulation summary diagram for dcuR

Alternative forms of DcuR transcriptional activator: DcuR

Gene Citations: [Salmon03]

Locations: cytosol

Map Position: [4,347,338 <- 4,348,057] (93.7 centisomes, 337°)
Length: 720 bp / 239 aa

Molecular Weight of Polypeptide: 27.488 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013507 , DIP:DIP-48032N , EchoBASE:EB2357 , EcoGene:EG12464 , EcoliWiki:b4124 , ModBase:P0AD01 , OU-Microarray:b4124 , PortEco:dcuR , PR:PRO_000022415 , Protein Model Portal:P0AD01 , RegulonDB:G7826 , SMR:P0AD01 , String:511145.b4124 , UniProt:P0AD01

Relationship Links: InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR024187 , Pfam:IN-FAMILY:PF00072 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

DcuSR Two-Component Signal Transduction System, dicarboxylate-dependent :
DcuR + DcuS sensory histidine kinase - phosphorylated → DcuR-Pasp + DcuS

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for dcuR

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11, Zientz98]
GO:0000160 - phosphorelay signal transduction system Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0009605 - response to external stimulus Inferred by computational analysis [GOA01]
Molecular Function: GO:0003677 - DNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Janausch02]
GO:0001071 - nucleic acid binding transcription factor activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA01]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism energy metabolism, carbon anaerobic respiration
regulation type of regulation transcriptional level activator
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for dcuR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of DcuR transcriptional activator with features indicated

Feature Class Location Citations Comment State
Conserved-Region 1 -> 121
UniProt: Response regulatory;
4-aspartylphosphate-Modification 56
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
DNA-Binding-Region 181 -> 200
UniProt: H-T-H motif;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Janausch02: Janausch IG, Garcia-Moreno I, Unden G (2002). "Function of DcuS from Escherichia coli as a fumarate-stimulated histidine protein kinase in vitro." J Biol Chem 2002;277(42);39809-14. PMID: 12167640

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Zientz98: Zientz E, Bongaerts J, Unden G (1998). "Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system." J Bacteriol 1998;180(20);5421-5. PMID: 9765574

Other References Related to Gene Regulation

AboAmer09: Abo-Amer AE, Altalhi AD (2009). "RpoS-independent and growth phase-dependent expression of dcuSR operon of Escherichia coli." Acta Microbiol Immunol Hung 56(3);211-27. PMID: 19789137

Goh05: Goh EB, Bledsoe PJ, Chen LL, Gyaneshwar P, Stewart V, Igo MM (2005). "Hierarchical control of anaerobic gene expression in Escherichia coli K-12: the nitrate-responsive NarX-NarL regulatory system represses synthesis of the fumarate-responsive DcuS-DcuR regulatory system." J Bacteriol 187(14);4890-9. PMID: 15995204

Oyamada07: Oyamada T, Yokoyama K, Morinaga M, Suzuki M, Makino K (2007). "Expression of Escherichia coli DcuS-R two-component regulatory system is regulated by the secondary internal promoter which is activated by CRP-cAMP." J Microbiol 45(3);234-40. PMID: 17618229

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Oct 5, 2015, biocyc13.