Escherichia coli K-12 substr. MG1655 Polypeptide: KpLE2 phage-like element; predicted DNA-binding transcriptional regulator

Gene: sgcR Accession Numbers: G7913 (EcoCyc), b4300, ECK4289

Synonyms: yjhJ

Regulation Summary Diagram: ?

Regulation summary diagram for sgcR

No information about this protein was found by a literature search conducted on October 11, 2011.

Locations: cytosol

Map Position: [4,524,129 <- 4,524,911] (97.51 centisomes, 351°)
Length: 783 bp / 260 aa

Molecular Weight of Polypeptide: 29.268 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014094 , DIP:DIP-10880N , EchoBASE:EB2440 , EcoGene:EG12552 , EcoliWiki:b4300 , ModBase:P39361 , OU-Microarray:b4300 , PortEco:sgcR , Pride:P39361 , Protein Model Portal:P39361 , RefSeq:NP_418720 , RegulonDB:G7913 , SMR:P39361 , String:511145.b4300 , UniProt:P39361

Relationship Links: InterPro:IN-FAMILY:IPR001034 , InterPro:IN-FAMILY:IPR014036 , InterPro:IN-FAMILY:IPR018356 , Pfam:IN-FAMILY:PF00455 , Pfam:IN-FAMILY:PF08220 , Prosite:IN-FAMILY:PS00894 , Prosite:IN-FAMILY:PS51000 , Smart:IN-FAMILY:SM00420

In Paralogous Gene Group: 583 (2 members)

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, PerezRueda00, Bairoch05, PerezRueda04]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005622 - intracellular Inferred by computational analysis [GOA01]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level repressor

Essentiality data for sgcR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 11-Oct-2011 by Keseler I , SRI International

Sequence Features

Protein sequence of KpLE2 phage-like element; predicted DNA-binding transcriptional regulator with features indicated

Feature Class Location Citations Comment
Conserved-Region 5 -> 61
UniProt: HTH deoR-type;
DNA-Binding-Region 22 -> 41
UniProt: H-T-H motif; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bairoch05: Bairoch A, Apweiler R, Wu CH, Barker WC, Boeckmann B, Ferro S, Gasteiger E, Huang H, Lopez R, Magrane M, Martin MJ, Natale DA, O'Donovan C, Redaschi N, Yeh LS (2005). "The Universal Protein Resource (UniProt)." Nucleic Acids Res 33(Database issue);D154-9. PMID: 15608167

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

PerezRueda00: Perez-Rueda E, Collado-Vides J (2000). "The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12." Nucleic Acids Res 28(8);1838-47. PMID: 10734204

PerezRueda04: Perez-Rueda E, Collado-Vides J, Segovia L (2004). "Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea." Comput Biol Chem 28(5-6);341-50. PMID: 15556475

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.