M9 medium is a defined, minimal medium for E. coli. It is one of the simplest media in common use, providing a bare-bones complement of phosphorous, nitrogen, and sulfur. It is the medium of choice for microscopy of E. coli as it generates significantly less autofluorescence than other commonly used media such as LB [Xiao07].
This entry describes M9 medium without a carbon source. Variations that describe M9 with a selected carbon source include M9 medium with 0.4% glucose, M9 medium with 1% glycerol, and M9 medium with 2% glycerol.
| Recipe Substances:
Osmolarity (approximate, computed from constituents): 0.24 Osm/L
Wildtype growth observations:
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