Escherichia coli K-12 substr. MG1655 Growth Medium: M9 medium base

M9 medium is a defined, minimal medium for E. coli. It is one of the simplest media in common use, providing a bare-bones complement of phosphorous, nitrogen, and sulfur. It is the medium of choice for microscopy of E. coli as it generates significantly less autofluorescence than other commonly used media such as LB [Xiao07].

This entry describes M9 medium without a carbon source. Variations that describe M9 with a selected carbon source include M9 medium with 0.4% glucose, M9 medium with 1% glycerol, and M9 medium with 2% glycerol.

Citations: [Maniatis82]

Recipe Substances:
Substances Concentration Role
disodium phosphate heptahydrate 12.8 g/l Source of P
monopotassium phosphate 3 g/l Source of P
ammonium chloride 1 g/l Source of N
sodium chloride 0.5 g/l  
magnesium sulfate 2 mM Source of S
Constituents Concentration
Na+ 103.7 mM
phosphate 69.454 mM
chloride 27.25 mM
K+ 21.883 mM
ammonium 18.695 mM
sulfate 2 mM
Mg2+ 2 mM

pH: 7.2

Osmolarity (approximate, computed from constituents): 0.24 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic No


Maniatis82: Maniatis, T., Fritsch, E.F., Sambrook, J. (1982). "Molecular cloning: a laboratory manual." Molecular cloning: a laboratory manual (CSHL Press).

Xiao07: Xiao, J, Elf, J, et al (2007). "Imaging gene expression in living cells at the single-molecule level." Single Molecules: A Laboratory Manual (CSHL Press).

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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