M9 medium is a defined, minimal medium for E. coli. It is one of the simplest media in common use, providing a bare-bones complement of phosphorous, nitrogen, and sulfur. It is the medium of choice for microscopy of E. coli as it generates significantly less autofluorescence than other commonly used media such as LB [Xiao07].
This entry describes M9 medium with .4% glucose as the carbon source.
| Recipe Substances:
Osmolarity (approximate, computed from constituents): 0.27 Osm/L
Wildtype growth observations:
106 single gene knockouts exhibit no growth (aerobic):
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