Escherichia coli K-12 substr. MG1655 Growth Medium: M9 medium with 0.4% glucose

M9 medium is a defined, minimal medium for E. coli. It is one of the simplest media in common use, providing a bare-bones complement of phosphorous, nitrogen, and sulfur. It is the medium of choice for microscopy of E. coli as it generates significantly less autofluorescence than other commonly used media such as LB [Xiao07].

This entry describes M9 medium with .4% glucose as the carbon source.

Citations: [Maniatis82]

Recipe Substances:
Substances Concentration Role
disodium phosphate heptahydrate 12.8 g/l Source of P
β-D-glucopyranose 4 g/l Source of C
monopotassium phosphate 3 g/l Source of P
ammonium chloride 1 g/l Source of N
sodium chloride 0.5 g/l  
magnesium sulfate 2 mM Source of S
Constituents Concentration
Na+ 103.7 mM
phosphate 69.454 mM
chloride 27.25 mM
β-D-glucopyranose 22.203 mM
K+ 21.883 mM
ammonium 18.695 mM
Mg2+ 2 mM
sulfate 2 mM

pH: 7.2

Osmolarity (approximate, computed from constituents): 0.27 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes

106 single gene knockouts exhibit no growth (aerobic):

argA , argB , argC , argE , argG , argH , aroA , aroB , aroC , aroE , bioA , bioB , bioC , bioD , bioF , bioH , carA , carB , cysB , cysC , cysD , cysE , cysG , cysH , cysI , cysJ , cysN , fes , glnA , gltA , glyA , guaA , guaB , hisA , hisB , hisC , hisD , hisF , hisG , hisH (T=37°C) [Patrick07, Comment 1] + 66 more...
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Maniatis82: Maniatis, T., Fritsch, E.F., Sambrook, J. (1982). "Molecular cloning: a laboratory manual." Molecular cloning: a laboratory manual (CSHL Press).

Patrick07: Patrick WM, Quandt EM, Swartzlander DB, Matsumura I (2007). "Multicopy suppression underpins metabolic evolvability." Mol Biol Evol 24(12);2716-22. PMID: 17884825

Xiao07: Xiao, J, Elf, J, et al (2007). "Imaging gene expression in living cells at the single-molecule level." Single Molecules: A Laboratory Manual (CSHL Press).

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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