Escherichia coli K-12 substr. MG1655 Growth Medium: MOPS medium base

Citations: [Neidhardt74]

Recipe Substances:
Substances Concentration Role
3-(N-morpholino)propanesulfonate 40 mM pH Buffer
ammonium chloride 9.5 mM Source of N
ammonium heptamolybdate 3 nM  
borate 400 nM  
calcium chloride 0.5 µM  
cobalt chloride 30.0 nM  
copper sulfate 10 nM  
dipotassium phosphate 1.32 mM Source of P
iron sulfate 10 µM  
manganese chloride 80 nM  
MgCl2 528 µM  
potassium sulfate 276 µM Source of S
sodium chloride 50 mM  
tricine 4 mM pH Buffer
zinc sulfate 10 nM  
Constituents Concentration
chloride 60.557 mM
Na+ 50 mM
3-(N-morpholino)propanesulfonate 40 mM
borate 24.732 mM
ammonium 9.5 mM
tricine 4 mM
K+ 3.192 mM
phosphate 1.32 mM
Mg2+ 528 µM
sulfate 286.02 µM
Fe2+ 10 µM
Ca2+ 500 nM
Mn2+ 80 nM
Co2+ 30.0 nM
Cu2+ 10 nM
Zn2+ 10 nM
heptamolybdate 3 nM

pH: 7.2

Osmolarity (approximate, computed from constituents): 0.19 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic No


Neidhardt74: Neidhardt FC, Bloch PL, Smith DF (1974). "Culture medium for enterobacteria." J Bacteriol 119(3);736-47. PMID: 4604283

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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