Escherichia coli K-12 substr. MG1655 Growth Medium: M63 medium with 2% glycerol

M63 is a defined minimal medium. It is similar in composition to M9 medium base by compound type. However, it has increased amounts of phosphate and ammonium relative to M9, and nearly an order of magnitude more sulfate and potassium. M63 also adds iron, which is not among the nutrients added to make M9.

This entry describes M63 minimal medium base with 2% glycerol as its carbon source.

Citations: [Pardee59]

Recipe Substances:
Substances Concentration Role
glycerol 20 g/l Source of C
ammonium sulfate 15 mM Source of N
iron sulfate 1.8 µM  
magnesium sulfate heptahydrate 1 mM Source of S
monopotassium phosphate 100 mM Source of P
Constituents Concentration
glycerol 217.17 mM
K+ 100 mM
phosphate 100 mM
ammonium 30 mM
sulfate 16.002 mM
Mg2+ 1 mM
Fe2+ 1.8 µM

pH: 7

Osmolarity (approximate, computed from constituents): 0.46 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes


Pardee59: Pardee AB, Jacob F, Monod J (1959). "The genetic control and cytoplasmic expression of "Inducibility" in the synthesis of β-galactosidase by E. coli." J Mol Biol 1(2), 165-178.

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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