Escherichia coli K-12 substr. MG1655 Growth Medium: PMA carbon source test + L-tartrate

Superclasses: Phenotype-Microarray-Media

Plate and Well ID: Biolog PM2 - Carbon Sources: F12

Citations: [Bochner01]

Recipe Substances:
Substances Concentration Role
ammonium chloride 5 mM Source of N
disodium phosphate 2 mM Source of P
ferric chloride 1 µM  
L-tartrate Source of C
MgCl2 50 µM  
potassium chloride 1 mM  
sodium chloride 100 mM  
sodium sulfate 250 µM Source of S
triethanolamine HCI 30 mM  
Constituents Concentration
chloride 136.1 mM
Na+ 104.5 mM
triethanolamine 30 mM
ammonium 5 mM
phosphate 2 mM
K+ 1 mM
sulfate 250 µM
Mg2+ 50 µM
Fe3+ 1 µM
L-tartrate [unknown]

Wildtype growth observations:

T (°C) O2 Growth Observations Consensus Comment/Citations
37 Aerobic No [AbuOun09]
Low [Bochner12]
No [Mackie12]
No [Yoon12]
No* Aerobic growth on L-tartrate has been reported for the E. coli K-12 derived strain W3110 and for 'coliform' bacteria. In E.coli K-12 MG1655 L-tartrate supported slow growth in M9 media enriched with amino acids but no growth in M9 medium without supplementation. [Reaney93, Vaughn46, Lukas10]

*A curator has resolved the inconsistency.


AbuOun09: AbuOun M, Suthers PF, Jones GI, Carter BR, Saunders MP, Maranas CD, Woodward MJ, Anjum MF (2009). "Genome scale reconstruction of a Salmonella metabolic model: comparison of similarity and differences with a commensal Escherichia coli strain." J Biol Chem 284(43);29480-8. PMID: 19690172

Bochner01: Bochner BR, Gadzinski P, Panomitros E (2001). "Phenotype microarrays for high-throughput phenotypic testing and assay of gene function." Genome Res 11(7);1246-55. PMID: 11435407

Bochner12: Bochner B (2012). Personal communication.

Lukas10: Lukas H, Reimann J, Kim OB, Grimpo J, Unden G (2010). "The regulation of aerobic and anaerobic D-malate metabolism of Escherichia coli by the LysR-type regulator DmlR (former YeaT)." J Bacteriol 192(10):2503-11. PMID: 20233924

Mackie12: Mackie A, Paulsen I (2012). Personal communication.

Reaney93: Reaney SK, Begg C, Bungard SJ, Guest JR (1993). "Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of Escherichia coli and evolutionary relationship with the class I fumarase genes." J Gen Microbiol 1993;139 ( Pt 7);1523-30. PMID: 8371115

Vaughn46: Vaughn RH, Marsh GL, Stadtman TC, Cantino BC (1946). "Decomposition of Tartrates by the Coliform Bacteria." J Bacteriol 52(3);311-25. PMID: 16561180

Yoon12: Yoon SH, Han MJ, Jeong H, Lee CH, Xia XX, Lee DH, Shim JH, Lee SY, Oh TK, Kim JF (2012). "Comparative multi-omics systems analysis of Escherichia coli strains B and K-12." Genome Biol 13(5);R37. PMID: 22632713

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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