Escherichia coli K-12 substr. MG1655 Growth Medium: PMA carbon source test + acetamide

Superclasses: Phenotype-Microarray-Media

Plate and Well ID: Biolog PM2 - Carbon Sources: G1

Citations: [Bochner01]

Recipe Substances:
Substances Concentration Role
acetamide Source of C
ammonium chloride 5 mM Source of N
disodium phosphate 2 mM Source of P
ferric chloride 1 µM  
MgCl2 50 µM  
potassium chloride 1 mM  
sodium chloride 100 mM  
sodium sulfate 250 µM Source of S
triethanolamine HCI 30 mM  
Constituents Concentration
chloride 136.1 mM
Na+ 104.5 mM
triethanolamine 30 mM
ammonium 5 mM
phosphate 2 mM
K+ 1 mM
sulfate 250 µM
Mg2+ 50 µM
Fe3+ 1 µM
acetamide [unknown]

Wildtype growth observations:

T (°C) O2 Growth Observations Consensus Comment/Citations
37 Aerobic No [AbuOun09]
Low [Bochner12]
No [Mackie12]
No [Yoon12]
No* E.coli is considered to be acetamide negative in diagnostic testing. [York07]

*A curator has resolved the inconsistency.


AbuOun09: AbuOun M, Suthers PF, Jones GI, Carter BR, Saunders MP, Maranas CD, Woodward MJ, Anjum MF (2009). "Genome scale reconstruction of a Salmonella metabolic model: comparison of similarity and differences with a commensal Escherichia coli strain." J Biol Chem 284(43);29480-8. PMID: 19690172

Bochner01: Bochner BR, Gadzinski P, Panomitros E (2001). "Phenotype microarrays for high-throughput phenotypic testing and assay of gene function." Genome Res 11(7);1246-55. PMID: 11435407

Bochner12: Bochner B (2012). Personal communication.

Mackie12: Mackie A, Paulsen I (2012). Personal communication.

Yoon12: Yoon SH, Han MJ, Jeong H, Lee CH, Xia XX, Lee DH, Shim JH, Lee SY, Oh TK, Kim JF (2012). "Comparative multi-omics systems analysis of Escherichia coli strains B and K-12." Genome Biol 13(5);R37. PMID: 22632713

York07: York MK, Traylor MM, Hardy J, Henry M (2007). "Biochemical tests for the identification of aerobic bacteria." In Clinical Microbiology Procedures Handbook (3rd ed.), Garcia LS (ed.), American Society for Microbiology, 2010, volume 1, section 3.17.1.

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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