Escherichia coli K-12 substr. MG1655 Growth Medium: AB medium with 0.4% acetate

AB medium is a derivative of M9 minimal medium that is intended to reduce issues with salt preciptation that can occur with M9. The composition in this entry represents AB medium with low-concentration acetate as the carbon source.

Recipe Substances:
Substances Concentration Role
disodium phosphate 6 g/l Source of P
acetate 4 g/l Source of C
monopotassium phosphate 3 g/l Source of P
sodium chloride 3 g/l  
ammonium sulfate 2 g/l Source of N S
calcium chloride 100 µM  
ferric chloride 3 µM  
MgCl2 1 mM  
Constituents Concentration
Na+ 135.27 mM
acetate 67.746 mM
phosphate 63.851 mM
chloride 53.541 mM
ammonium 30.272 mM
K+ 21.883 mM
sulfate 15.136 mM
Mg2+ 1 mM
Ca2+ 100 µM
Fe3+ 3 µM

Osmolarity (approximate, computed from constituents): 0.39 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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