Escherichia coli K-12 substr. MG1655 Growth Medium: M9 medium with 2% glycerol

M9 medium is a defined, minimal medium for E. coli. It is one of the simplest media in common use, providing a bare-bones complement of phosphorous, nitrogen, and sulfur. It is the medium of choice for microscopy of E. coli as it generates significantly less autofluorescence than other commonly used media such as LB [Xiao07].

This entry describes M9 medium with 2% glycerol as the carbon source.

Citations: [Maniatis82]

Recipe Substances:
Substances Concentration Role
glycerol 20 g/l Source of C
disodium phosphate heptahydrate 12.8 g/l Source of P
monopotassium phosphate 3 g/l Source of P
ammonium chloride 1 g/l Source of N
sodium chloride 0.5 g/l  
magnesium sulfate 2 mM Source of S
Constituents Concentration
glycerol 217.17 mM
Na+ 103.7 mM
phosphate 69.454 mM
chloride 27.25 mM
K+ 21.883 mM
ammonium 18.695 mM
Mg2+ 2 mM
sulfate 2 mM

pH: 7.2

Osmolarity (approximate, computed from constituents): 0.46 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes


Maniatis82: Maniatis, T., Fritsch, E.F., Sambrook, J. (1982). "Molecular cloning: a laboratory manual." Molecular cloning: a laboratory manual (CSHL Press).

Xiao07: Xiao, J, Elf, J, et al (2007). "Imaging gene expression in living cells at the single-molecule level." Single Molecules: A Laboratory Manual (CSHL Press).

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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