Escherichia coli K-12 substr. MG1655 Growth Medium: ATCC medium 57

This medium is used for cultivation and maintenance of Escherchia coli.

Recipe Substances:
Substances Concentration Role
dipotassium phosphate 7 g/l  
glycerol 5 g/l Source of C
monopotassium phosphate 3 g/l Source of P
ammonium sulfate 1.5 g/l Source of N S
ferrous sulfate heptahydrate 500 mg/l  
L-lysine 100 mg/l  
magnesium sulfate 100 mg/l Source of S
calcium chloride 10 mg/l  
Constituents Concentration
K+ 101.8 mM
phosphate 61.841 mM
glycerol 54.292 mM
ammonium 22.704 mM
sulfate 13.981 mM
Fe2+ 1.7985 mM
Mg2+ 830.82 µM
L-lysine 679.36 µM
chloride 180.2 µM
Ca2+ 90.101 µM

pH: 7.1

Osmolarity (approximate, computed from constituents): 0.26 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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