Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Growth Medium: MOPS medium with 2% glycerol

Recipe Substances: ?
Substances Concentration Role
glycerol 20 g/l Source of C
3-(N-morpholino)propanesulfonate 40 mM pH Buffer
ammonium chloride 9.5 mM Source of N
ammonium heptamolybdate 3 nM  
borate 400 nM  
calcium chloride 0.5 µM  
cobalt chloride 30.0 nM  
copper sulfate 10 nM  
dipotassium phosphate 1.32 mM Source of P
iron sulfate 10 µM  
manganese chloride 80 nM  
MgCl2 528 µM  
potassium sulfate 276 µM Source of S
sodium chloride 50 mM  
tricine 4 mM pH Buffer
zinc sulfate 10 nM  
Composition: ?
Constituents Concentration
glycerol 217.17 mM
chloride 60.557 mM
Na+ 50 mM
3-(N-morpholino)propanesulfonate 40 mM
borate 24.732 mM
ammonium 9.5 mM
tricine 4 mM
K+ 3.192 mM
phosphate 1.32 mM
Mg2+ 528 µM
sulfate 286.02 µM
Fe2+ 10 µM
Ca2+ 500 nM
Mn2+ 80 nM
Co2+ 30.0 nM
Zn2+ 10 nM
Cu2+ 10 nM
heptamolybdate 3 nM

pH: 7.2

Osmolarity (approximate, computed from constituents): 0.41 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC13A.