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Escherichia coli K-12 substr. MG1655 Growth Medium: Neidhardt EZ rich defined medium

Citations: [Neidhardt74]

Recipe Substances: ?
Substances Concentration Role
2,3-dihydroxybenzoate 0.01 mM  
3-(N-morpholino)propanesulfonate 40 mM pH Buffer
4-aminobenzoate 0.01 mM  
4-hydroxybenzoate 0.01 mM  
adenine 0.2 mM  
ammonium chloride 9.5 mM Source of N
ammonium heptamolybdate 3 nM  
borate 0.4 µM  
calcium chloride 0.5 µM  
calcium pantothenate 0.01 mM  
cobalt chloride 30.0 nM  
copper sulfate 10 nM  
cytosine 0.2 mM  
dipotassium phosphate 1.32 mM Source of P
glucose 19.84 mM Source of C
glycine 0.8 mM  
guanine 0.2 mM  
iron sulfate 10 µM  
L-alanine 0.8 mM  
L-arginine 5.2 mM  
L-asparagine 0.4 mM  
L-aspartate 0.4 mM  
L-cysteine 0.1 mM  
L-glutamate 0.6 mM  
L-glutamine 0.6 mM  
L-histidine 0.2 mM  
L-isoleucine 0.4 mM  
L-leucine 0.8 mM  
L-lysine 0.4 mM  
L-methionine 0.2 mM  
L-phenylalanine 0.4 mM  
L-proline 0.4 mM  
L-serine 10 mM  
L-threonine 0.4 mM  
L-tryptophan 0.1 mM  
L-tyrosine 0.2 mM  
L-valine 0.6 mM  
manganese chloride 80 nM  
MgCl2 528 µM  
potassium sulfate 276 µM Source of S
sodium chloride 50 mM  
thiamin 0.01 mM  
tricine 4 mM pH Buffer
uracil 0.2 mM  
zinc sulfate 10 nM  
Composition: ?
Constituents Concentration
chloride 60.557 mM
Na+ 50 mM
3-(N-morpholino)propanesulfonate 40 mM
borate 24.732 mM
glucose 19.84 mM
L-serine 10 mM
ammonium 9.5 mM
L-arginine 5.2 mM
tricine 4 mM
K+ 3.192 mM
phosphate 1.32 mM
L-alanine 800 µM
L-leucine 800 µM
glycine 800 µM
L-glutamate 600 µM
L-glutamine 600 µM
L-valine 600 µM
Mg2+ 528 µM
L-proline 400 µM
L-isoleucine 400 µM
L-threonine 400 µM
L-asparagine 400 µM
L-aspartate 400 µM
L-lysine 400 µM
L-phenylalanine 400 µM
sulfate 286.02 µM
L-histidine 200 µM
adenine 200 µM
guanine 200 µM
cytosine 200 µM
uracil 200 µM
L-methionine 200 µM
L-tyrosine 200 µM
L-cysteine 100 µM
L-tryptophan 100 µM
(R)-pantothenate 20 µM
Ca2+ 10.5 µM
Fe2+ 10 µM
thiamin 10 µM
4-aminobenzoate 10 µM
4-hydroxybenzoate 10 µM
2,3-dihydroxybenzoate 10 µM
Mn2+ 80 nM
Co2+ 30.0 nM
Zn2+ 10 nM
Cu2+ 10 nM
heptamolybdate 3 nM

pH: 7.2

Osmolarity (approximate, computed from constituents): 0.24 Osm/L

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes

References

Neidhardt74: Neidhardt FC, Bloch PL, Smith DF (1974). "Culture medium for enterobacteria." J Bacteriol 119(3);736-47. PMID: 4604283


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc13.