Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Growth Medium: PMA nitrogen source test + gly-met

Superclasses: Phenotype-Microarray-Media

Plate and Well ID: Biolog PM3 - Nitrogen Sources: H11

Citations: [Bochner01]

Recipe Substances: ?
Substances Concentration Role
disodium phosphate 2 mM Source of P
ferric chloride 1 µM  
glycyl-L-methionine Source of N
MgCl2 50 µM  
potassium chloride 1 mM  
sodium chloride 100 mM  
sodium pyruvate 25 mM Source of C
sodium sulfate 250 µM Source of S
triethanolamine HCI 30 mM  
Composition: ?
Constituents Concentration
chloride 131.1 mM
Na+ 129.5 mM
triethanolamine 30 mM
pyruvate 25 mM
phosphate 2 mM
K+ 1 mM
sulfate 250 µM
Mg2+ 50 µM
Fe3+ 1 µM
glycyl-L-methionine [unknown]

Wildtype growth observations:

T (°C) O2 Growth Observations Consensus
37 Aerobic Yes [AbuOun09]
Yes [Bochner12]
Yes [Yoon12]
Yes [Mackie12]
Yes

References

AbuOun09: AbuOun M, Suthers PF, Jones GI, Carter BR, Saunders MP, Maranas CD, Woodward MJ, Anjum MF (2009). "Genome scale reconstruction of a Salmonella metabolic model: comparison of similarity and differences with a commensal Escherichia coli strain." J Biol Chem 284(43);29480-8. PMID: 19690172

Bochner01: Bochner BR, Gadzinski P, Panomitros E (2001). "Phenotype microarrays for high-throughput phenotypic testing and assay of gene function." Genome Res 11(7);1246-55. PMID: 11435407

Bochner12: Bochner B (2012). Personal communication.

Mackie12: Mackie A, Paulsen I (2012). Personal communication.

Yoon12: Yoon SH, Han MJ, Jeong H, Lee CH, Xia XX, Lee DH, Shim JH, Lee SY, Oh TK, Kim JF (2012). "Comparative multi-omics systems analysis of Escherichia coli strains B and K-12." Genome Biol 13(5);R37. PMID: 22632713


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14A.