Escherichia coli K-12 substr. MG1655 Growth Medium: LB Lennox

Lysogeny broth (LB) is a nutritionally rich medium, primarily used for the growth of bacteria. LB media formulations have been an industry standard for the cultivation of Escherichia coli since the 1950s. In his original 1951 paper, Bertani used 10 g/L NaCl and 1 g/L glucose [Bertani51]. Recipes published later have typically omitted the glucose and varied the amount of NaCl. The LB Lennox broth is a lower-salt variation.

Citations: [Lennox55]

Recipe Substances:
Substances Concentration Role
tryptone 10 g/l Source of C N P S
yeast extract 5 g/l Source of C N P S
sodium chloride 5 g/l  
Constituents Concentration
Na+ 85.553 mM
chloride 85.553 mM
yeast extract

pH: 7

Wildtype growth observations:

T (°C) O2 Growth?
37 Aerobic Yes [Lennox55]

318 single gene knockouts exhibit no growth (aerobic):

alaS , btuB , coaA , coaE , djlB , dnaG , folP , glmM , glyS , groL , hemE , ileS , lptB , parC , polA , prfB , priB , rho , rplK , rpoD , rpsU , tpr , yiaD (T=37°C) [Baba06, Yamamoto09],
accA , accB , accC , accD , acpP , acpS , adk , alsK , argS , asd , asnS , aspS , bamA , bamD , bcsB , birA , can (T=37°C) [Baba06, Comment 1] + 278 more...
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3840 single gene knockouts exhibit growth (aerobic):

aaeA , aaeB , aaeR , aaeX , aas , aat , abgA , abgB , abgR , abgT , abpA , abpB , abrB , aceA , aceB , aceE , aceF , aceK , ackA , acnA , acnB , acpH , acpT , acrA , acrB , acrD , acrE , acrF , acrR , acrZ , acs , actP , ada , add , adeD , adeP , adeQ , adhE , adhP , adiA (T=37°C) [Baba06, Comment 2] + 3800 more...
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Genes with inconsistent growth observations:

Knockout Growth? T (°C) O2 Citations Growth Observations
efpLow 37 Aerobic [Yanagisawa10]
No [Aoki97]
Yes [Baba06, Comment 2]
lapBIndeterminate 37 Aerobic  
No [Mahalakshmi14]
Yes [Baba06, Comment 2]
nusBIndeterminate 37 Aerobic  
Low [Bubunenko07]
Yes [Baba06, Comment 2]
rpsFIndeterminate 37 Aerobic  
Low [Bubunenko07]
Yes [Baba06, Comment 2]
rpsIIndeterminate 37 Aerobic  
No [Baba06, Comment 1]
Low [Bubunenko07]
rpsMIndeterminate 37 Aerobic  
No [Baba06, Comment 1]
Low [Bubunenko07]
rpsOIndeterminate 37 Aerobic  
Low [Bubunenko07]
No [Baba06, Yamamoto09]
rpsQIndeterminate 37 Aerobic  
No [Baba06, Comment 1]
Low [Bubunenko07]
rpsTIndeterminate 37 Aerobic  
Low [Bubunenko07]
Yes [Baba06, Comment 2]
ubiJIndeterminate 37 Aerobic  
No [Baba06, Comment 1]
Yes [Aussel14]

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Aoki97: Aoki H, Dekany K, Adams SL, Ganoza MC (1997). "The gene encoding the elongation factor P protein is essential for viability and is required for protein synthesis." J Biol Chem 272(51);32254-9. PMID: 9405429

Aussel14: Aussel L, Loiseau L, Hajj Chehade M, Pocachard B, Fontecave M, Pierrel F, Barras F (2014). "ubiJ, a New Gene Required for Aerobic Growth and Proliferation in Macrophage, Is Involved in Coenzyme Q Biosynthesis in Escherichia coli and Salmonella enterica Serovar Typhimurium." J Bacteriol 196(1);70-9. PMID: 24142253

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bertani51: Bertani G (1951). "Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli." J Bacteriol 62(3);293-300. PMID: 14888646

Bubunenko07: Bubunenko M, Baker T, Court DL (2007). "Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli." J Bacteriol 189(7);2844-53. PMID: 17277072

Lennox55: Lennox ES (1955). "Transduction of linked genetic characters of the host by bacteriophage P1." Virology 1(2);190-206. PMID: 13267987

Mahalakshmi14: Mahalakshmi S, Sunayana MR, SaiSree L, Reddy M (2014). "yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli." Mol Microbiol 91(1);145-57. PMID: 24266962

Yamamoto09: Yamamoto N, Nakahigashi K, Nakamichi T, Yoshino M, Takai Y, Touda Y, Furubayashi A, Kinjyo S, Dose H, Hasegawa M, Datsenko KA, Nakayashiki T, Tomita M, Wanner BL, Mori H (2009). "Update on the Keio collection of Escherichia coli single-gene deletion mutants." Mol Syst Biol 5;335. PMID: 20029369

Yanagisawa10: Yanagisawa T, Sumida T, Ishii R, Takemoto C, Yokoyama S (2010). "A paralog of lysyl-tRNA synthetase aminoacylates a conserved lysine residue in translation elongation factor P." Nat Struct Mol Biol 17(9);1136-43. PMID: 20729861

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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