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Escherichia coli K-12 substr. MG1655 Enzyme: sugar phosphatase



Gene: yidA Accession Numbers: EG11195 (EcoCyc), b3697, ECK3689

Regulation Summary Diagram: ?

Summary:
YidA is a promiscuous sugar phosphatase belonging to the superfamily of haloacid dehalogenase (HAD)-like hydrolases. Its preferred substrate is erythrose-4-phosphate [Kuznetsova06]. YidA selectively hydrolyzes α-D-glucose-1-phosphate and has no activity with the β form [Kuznetsova06]. The phosphatase activity of YidA was first discovered in a high-throughput screen of purified proteins [Kuznetsova05].

Mutagenesis of the predicted catalytic Asp residues in YidA results in loss of phosphatase activity [Kuznetsova06].

Locations: cytosol

Map Position: [3,874,163 <- 3,874,975] (83.5 centisomes)
Length: 813 bp / 270 aa

Molecular Weight of Polypeptide: 29.721 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012086 , DIP:DIP-36033N , EchoBASE:EB1181 , EcoGene:EG11195 , EcoliWiki:b3697 , Mint:MINT-1238706 , ModBase:P0A8Y5 , OU-Microarray:b3697 , PortEco:yidA , Pride:P0A8Y5 , Protein Model Portal:P0A8Y5 , RefSeq:NP_418152 , RegulonDB:EG11195 , SMR:P0A8Y5 , String:511145.b3697 , Swiss-Model:P0A8Y5 , UniProt:P0A8Y5

Relationship Links: InterPro:IN-FAMILY:IPR000150 , InterPro:IN-FAMILY:IPR006379 , InterPro:IN-FAMILY:IPR023214 , PDB:Structure:1RKQ , Pfam:IN-FAMILY:PF00702 , Prosite:IN-FAMILY:PS01228 , Prosite:IN-FAMILY:PS01229

In Paralogous Gene Group: 129 (5 members)

Gene-Reaction Schematic: ?

Instance reaction of [D-glucopyranose 6-phosphate[periplasmic space] + H2O[periplasmic space] → D-glucopyranose[periplasmic space] + phosphate[periplasmic space]] (3.1.3.58):
i1: α-D-glucose 6-phosphate + H2O → α-D-glucose + phosphate (3.1.3.58)

Instance reactions of [a sugar phosphate + H2O → a sugar + phosphate] (3.1.3.23):
i2: β-D-glucose 6-phosphate + H2O → β-D-glucose + phosphate (3.1.3.58)

i3: β-D-fructofuranose 1-phosphate + H2O → β-D-fructofuranose + phosphate (3.1.3.-)

GO Terms:

Biological Process: GO:0016311 - dephosphorylation Inferred by computational analysis Inferred from experiment [Kuznetsova06, GOA01]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
GO:0044283 - small molecule biosynthetic process Inferred by computational analysis [Gaudet10]
Molecular Function: GO:0000287 - magnesium ion binding Inferred from experiment [Kuznetsova06]
GO:0016791 - phosphatase activity Inferred from experiment [Kuznetsova06]
GO:0050308 - sugar-phosphatase activity Inferred from experiment Inferred by computational analysis [GOA01, Kuznetsova06]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, LopezCampistrou05]
GO:0005737 - cytoplasm Inferred by computational analysis [Gaudet10]

Gene Class: UNCLASSIFIED

Essentiality data for yidA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Curated 02-Oct-2006 by Keseler I , SRI International
Last-Curated ? 17-Mar-2010 by Fulcher C , SRI International


Enzymatic reaction of: sugar phosphatase

Synonyms: HAD13

EC Number: 3.1.3.23

a sugar phosphate + H2O <=> a sugar + phosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for a sugar phosphate: imidodiphosphate [Kuznetsova06 ]

Summary:
Kms for the various sugar phosphate substrates range between 19 and 810 µM [Kuznetsova06].

Cofactors or Prosthetic Groups: Mg2+ [Kuznetsova06]


Sequence Features

Feature Class Location Citations Comment
Mutagenesis-Variant 9
[Kuznetsova06, UniProt12a]
Alternate sequence: D → A; UniProt: Loss of the phosphatase activity.
Metal-Binding-Site 9
[UniProt12a]
UniProt: Magnesium.
Protein-Segment 9 -> 11
[UniProt12a]
UniProt: Substrate; Sequence Annotation Type: region of interest; Non-Experimental Qualifier: by similarity.
Active-Site 9
[UniProt10]
UniProt: Nucleophile; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 11
[UniProt12a]
UniProt: Magnesium.
Metal-Binding-Site 220
[UniProt12a]
UniProt: Magnesium.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b3697 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11195; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kuznetsova05: Kuznetsova E, Proudfoot M, Sanders SA, Reinking J, Savchenko A, Arrowsmith CH, Edwards AM, Yakunin AF (2005). "Enzyme genomics: Application of general enzymatic screens to discover new enzymes." FEMS Microbiol Rev 29(2);263-79. PMID: 15808744

Kuznetsova06: Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF (2006). "Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family." J Biol Chem 281(47):36149-61. PMID: 16990279

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt12a: UniProt Consortium (2012). "UniProt version 2012-09 released on 2012-09-12 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc13.