Escherichia coli K-12 substr. MG1655 Transcription Unit: hisLGDCBHAFI
Inferred from experiment

Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Unit

Transcription-unit diagram


Other Transcription-Units in the Same Operon: hisL (hisLp), hisBHAFI (hisBp)

The hisLGDCBHAFI operon codes for the enzymes of the histidine biosynthesis pathway. It is controlled by attenuation in response to histidine availability. When histidine is abundant, the ribosome quickly translates through a stretch of seven histidine codons in the hisL leader region, allowing formation of the transcriptional terminator between hisL and hisG. In contrast, when histidine is not available, the ribosome stalls within the leader region and the terminator will not form, allowing transcription to continue [Di78, Verde81, Frunzio81].

Citations: [Alifano92]

Inferred from experimentInferred by computational analysis

Synonyms: hisGp

Guanosine tetraphosphate (ppGpp) increases the transcription of the hisLp promoter. In contrast, ppGpp reduces the transcription of other promoters, such as argTp and rrnBp1.

Citations: [Verde81]

Absolute Position of Transcription Start Site: 2087989 base pairs

Position relative to start of first gene (hisL): -31


Promoter sequence diagram

Regulation by allosteric regulation of RNA-Polymerase (alarmone-based regulation):

DksA-ppGpp activates transcription [Lee12b]
Inferred from experiment


Alifano92: Alifano P, Carlomagno MS, Bruni CB (1992). "Location of the hisGDCBHAFI operon on the physical map of Escherichia coli." J Bacteriol 1992;174(11);3830-1. PMID: 1592835

Di78: Di Nocera PP, Blasi F, Di Lauro R, Frunzio R, Bruni CB (1978). "Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12." Proc Natl Acad Sci U S A 75(9);4276-80. PMID: 360215

Frunzio81: Frunzio R, Bruni CB, Blasi F (1981). "In vivo and in vitro detection of the leader RNA of the histidine operon of Escherichia coli K-12." Proc Natl Acad Sci U S A 78(5);2767-71. PMID: 6166940

Goldschmidt70: Goldschmidt EP, Cater MS, Matney TS, Butler MA, Greene A (1970). "Genetic analysis of the histidine operon in Escherichia coli K12." Genetics 66(2);219-29. PMID: 4934197

Lee12b: Lee JH, Lennon CW, Ross W, Gourse RL (2012). "Role of the coiled-coil tip of Escherichia coli DksA in promoter control." J Mol Biol 416(4);503-17. PMID: 22200485

Verde81: Verde P, Frunzio R, di Nocera PP, Blasi F, Bruni CB (1981). "Identification, nucleotide sequence and expression of the regulatory region of the histidine operon of Escherichia coli K-12." Nucleic Acids Res 9(9);2075-86. PMID: 6170941

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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