Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites MAYBE down
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites MAYBE down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites MAYBE down
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Escherichia coli K-12 substr. MG1655 Transcription Unit: hisLGDCBHAFI

Local Context (not to scale): ?

Transcription Unit:

Notes: ?

Other Transcription-Units in the Same Operon: hisL (hisLp) , hisBHAFI (hisBp)

Summary:
The hisLGDCBHAFI operon codes for the enzymes of the histidine biosynthesis pathway. It is controlled by attenuation in response to histidine availability. When histidine is abundant, the ribosome quickly translates through a stretch of seven histidine codons in the hisL leader region, allowing formation of the transcriptional terminator between hisL and hisG. In contrast, when histidine is not available, the ribosome stalls within the leader region and the terminator will not form, allowing transcription to continue [Di78, Verde81, Frunzio81].

Citations: [Alifano92]


Promoter:

Synonyms: hisGp

Summary:
Guanosine tetraphosphate (ppGpp) increases the transcription of the hisLp promoter. In contrast, ppGpp reduces the transcription of other promoters, such as argTp and rrnBp1.

Citations: [Verde81]

Absolute Position of Transcription Start Site: 2087989 base pairs

Position relative to start of first gene (hisL): -31

Sequence:


Regulation by allosteric regulation of RNA-Polymerase (alarmone-based regulation):

DksA-ppGpp activates transcription [Lee12]

References

Alifano92: Alifano P, Carlomagno MS, Bruni CB (1992). "Location of the hisGDCBHAFI operon on the physical map of Escherichia coli." J Bacteriol 1992;174(11);3830-1. PMID: 1592835

Di78: Di Nocera PP, Blasi F, Di Lauro R, Frunzio R, Bruni CB (1978). "Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12." Proc Natl Acad Sci U S A 75(9);4276-80. PMID: 360215

Frunzio81: Frunzio R, Bruni CB, Blasi F (1981). "In vivo and in vitro detection of the leader RNA of the histidine operon of Escherichia coli K-12." Proc Natl Acad Sci U S A 78(5);2767-71. PMID: 6166940

Goldschmidt70: Goldschmidt EP, Cater MS, Matney TS, Butler MA, Greene A (1970). "Genetic analysis of the histidine operon in Escherichia coli K12." Genetics 66(2);219-29. PMID: 4934197

Lee12: Lee JH, Lennon CW, Ross W, Gourse RL (2012). "Role of the coiled-coil tip of Escherichia coli DksA in promoter control." J Mol Biol 416(4);503-17. PMID: 22200485

Verde81: Verde P, Frunzio R, di Nocera PP, Blasi F, Bruni CB (1981). "Identification, nucleotide sequence and expression of the regulatory region of the histidine operon of Escherichia coli K-12." Nucleic Acids Res 9(9);2075-86. PMID: 6170941


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 26, 2014, BIOCYC13B.