Escherichia coli K-12 substr. MG1655 Pathway: Entner-Doudoroff pathway I
Traceable author statement to experimental support

Pathway diagram: Entner-Doudoroff pathway I

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for Entner-Doudoroff pathway I

Synonyms: ED pathway

Superclasses: Degradation/Utilization/AssimilationCarbohydrates DegradationSugars DegradationEntner-Duodoroff Pathways
Generation of Precursor Metabolites and EnergyEntner-Duodoroff Pathways

The Entner-Doudoroff pathway joins the pentose phosphate pathway (oxidative branch) to glycolysis via two steps, catalyzed by inducible enzymes.

This pathway is important to E. coli because sugar acids, which are significant nutrients in both of its habitats, intestinal and aquatic, are metabolized via this pathway. Strains that are mutationally blocked in this pathway are unable to grow on gluconate, glucuronate, or galacturonate and are also unable to colonize the mouse intestine [Sweeney96]. The uronic acids feed into the pathway's sole intermediate, 2-keto-3-deoxy-6-phospho-gluconate so the dependence of their metabolism on an intact Entner-Doudoroff pathway is clear [Peekhaus98]. The dependence of gluconate's metabolism on an intact Entner-Doudoroff pathway is less obvious because it is also metabolized via the pentose phosphate pathway.

Enzymes of the Entner-Doudoroff pathway are induced by growth on gluconate, glucuronate, or methyl-β-D-glucuronide; phosphate or carbon limitation [Murray05].

Superpathways: superpathway of glycolysis and Entner-Doudoroff

Created 04-Apr-1994 by Riley M, Marine Biological Laboratory
Revised 09-May-2006 by Ingraham JL, UC Davis


Murray05: Murray EL, Conway T (2005). "Multiple regulators control expression of the Entner-Doudoroff aldolase (Eda) of Escherichia coli." J Bacteriol 187(3);991-1000. PMID: 15659677

Peekhaus98: Peekhaus N, Conway T (1998). "What's for dinner?: Entner-Doudoroff metabolism in Escherichia coli." J Bacteriol 1998;180(14);3495-502. PMID: 9657988

Sweeney96: Sweeney NJ, Laux DC, Cohen PS (1996). "Escherichia coli F-18 and E. coli K-12 eda mutants do not colonize the streptomycin-treated mouse large intestine." Infect Immun 64(9);3504-11. PMID: 8751891

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Egan92: Egan SE, Fliege R, Tong S, Shibata A, Wolf RE, Conway T (1992). "Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon." J Bacteriol 1992;174(14);4638-46. PMID: 1624451

Eisenberg67: Eisenberg RC, Dobrogosz WJ (1967). "Gluconate metabolism in Escherichia coli." J Bacteriol 1967;93(3);941-9. PMID: 5337840

Fradkin71: Fradkin JE, Fraenkel DG (1971). "2-keto-3-deoxygluconate 6-phosphate aldolase mutants of Escherichia coli." J Bacteriol 108(3);1277-83. PMID: 4945194

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Patil92: Patil RV, Dekker EE (1992). "Cloning, nucleotide sequence, overexpression, and inactivation of the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene." J Bacteriol 1992;174(1);102-7. PMID: 1339418

Pouyssegur71: Pouyssegur JM, Stoeber FR (1971). "[The common degradative pathway for hexuronates in Escherichia coli K 12. Purification, properties and individuality of 2-keto-3-deoxy-6-phospho-D-gluconate aldolase]." Eur J Biochem 1971;21(3);363-73. PMID: 4936448

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

VanBogelen92: VanBogelen RA, Sankar P, Clark RL, Bogan JA, Neidhardt FC (1992). "The gene-protein database of Escherichia coli: edition 5." Electrophoresis 1992;13(12);1014-54. PMID: 1286664

Walters08: Walters MJ, Srikannathasan V, McEwan AR, Naismith JH, Fierke CA, Toone EJ (2008). "Characterization and crystal structure of Escherichia coli KDPGal aldolase." Bioorg Med Chem 16(2):710-20. PMID: 17981470

Wang81: Wang JK, Dekker EE, Lewinski ND, Winter HC "Physical and chemical evidence for the trimeric subunit structure of 2-keto-4-hydroxyglutarate aldolase from Escherichia coli K-12." J Biol Chem 1981;256:1793-1800.

Zablotny67: Zablotny R, Fraenkel DG (1967). "Glucose and gluconate metabolism in a mutant of Escherichia coli lacking gluconate-6-phosphate dehydrase." J Bacteriol 93(5);1579-81. PMID: 5337844

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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