Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Pathway: D-galacturonate degradation I

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Genetic Regulation Schematic: ?

Synonyms: D-galacturonate catabolism, D-galacturonate degradation

Superclasses: Degradation/Utilization/Assimilation Carboxylates Degradation Sugar Acids Degradation D-Galacturonate Degradation
Degradation/Utilization/Assimilation Secondary Metabolites Degradation Sugar Derivatives Degradation Sugar Acids Degradation D-Galacturonate Degradation

Summary:
E. coli can use the hexuronate D-galacturonate as the sole sources of carbon for growth. The enzymes of this pathway were first described in a series of publications by Ashwell et al. [Ashwell58, Ashwell60, Hickman60, Smiley60, Cynkin60].

The initial step in the degradation of D-galacturonate is its isomerization to D-tagaturonate by D-galacturonate isomerase, an enzyme which can carry out the analogous function in the superpathway of β-D-glucuronide and D-glucuronate degradation pathway. D-tagaturonate then undergoes an NADH-dependent reduction to D-altronate by altronate oxidoreductase. D-altronate dehydratase subsequently catalyzes dehydration to yield 2-dehydro-3-deoxy-D-gluconate. At this point, the superpathway of β-D-glucuronide and D-glucuronate degradation and D-galacturonate degradation I pathways converge, and a common enzyme, 2-keto-3-deoxygluconokinase, phosphorylates 2-dehydro-3-deoxy-D-gluconate to yield 2-dehydro-3-deoxy-D-gluconate-6-phosphate, which enters central metabolism via the Entner-Doudoroff pathway I.

Review: Mandrand-Berthelot, M.-A., Condemine, G., and Hugouvieux-Cotte-Pattat, N., EcoSal Module 3.4.2 [ECOSAL]

Superpathways: superpathway of hexuronide and hexuronate degradation

Credits:
Created 09-Sep-1994 by Riley M , Marine Biological Laboratory
Last-Curated ? 01-Feb-2008 by Keseler I , SRI International


References

Ashwell58: Ashwell A, Wahba AJ, Hickman J (1958). "A new pathway of uronic acid metabolism." Biochim Biophys Acta 30(1);186-7. PMID: 13584413

Ashwell60: Ashwell G, Wahba AJ, Hickman J (1960). "Uronic acid metabolism in bacteria. I. Purification and properties of uronic acid isomerase in Escherichia coli." J Biol Chem 1960;235(6):1559-1565. PMID: 13794771

Cynkin60: Cynkin MA, Ashwell G (1960). "Uronic acid metabolism in bacteria. IV. Purification and properties of 2-keto-3-deoxy-D-gluconokinase in Escherichia coli." J Biol Chem 235;1576-9. PMID: 13813474

ECOSAL: "Escherichia coli and Salmonella: Cellular and Molecular Biology." Online edition.

Hickman60: Hickman J, Ashwell G (1960). "Uronic acid metabolism in bacteria. II. Purification and properties of D-altronic acid and D-mannonic acid dehydrogenases in Escherichia coli." J Biol Chem 235;1566-70. PMID: 14401695

Smiley60: Smiley JD, Ashwell G (1960). "Uronic acid metabolism in bacteria. III. Purification and properties of D-altronic acid and D-mannonic acid dehydrases in Escherichia coli." J Biol Chem 235;1571-5. PMID: 13831814

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Blanco83: Blanco C, Mata-Gilsinger M, Ritzenthaler P (1983). "Construction of hybrid plasmids containing the Escherichia coli uxaB gene: analysis of its regulation and direction of transcription." J Bacteriol 153(2);747-55. PMID: 6296052

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dreyer87: Dreyer JL (1987). "The role of iron in the activation of mannonic and altronic acid hydratases, two Fe-requiring hydro-lyases." Eur J Biochem 166(3);623-30. PMID: 3038546

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

HugouvieuxCotte81: Hugouvieux-Cotte-Pattat N, Robert-Baudouy J (1981). "Isolation of fusions between the lac genes and several genes of the exu regulon: analysis of their regulation, determination of the transcription direction of the uxaC-uxaA operon, in Escherichia coli K-12." Mol Gen Genet 182(2);279-87. PMID: 7026975

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Linster04: Linster CL, Van Schaftingen E (2004). "A spectrophotometric assay of D-glucuronate based on Escherichia coli uronate isomerase and mannonate dehydrogenase." Protein Expr Purif 37(2);352-60. PMID: 15358357

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Novel73: Novel G, Stoeber F (1973). "[Individuality of D glucuronate-ketol isomerase from K 12 Escherichia coli]." Biochimie 55(9);1057-70. PMID: 4594308

Portalier72: Portalier RC, Stoeber FR (1972). "[D-altronate: NAD-oxidoreductase in Escherichia coli K12. Purification, properties, and specificity]." Eur J Biochem 1972;26(1);50-61. PMID: 4402917

Portalier72a: Portalier RC (1972). "[D-altronate: NAD-oxidoreductase from Escherichia coli K12. Kinetic studies]." Eur J Biochem 1972;30(2);211-9. PMID: 4351434

Portalier72b: Portalier RC, Stoeber FR (1972). "[Colorimetric determinations of aldonate oxidoreductases of Escherichia coli K 12: applications]." Biochim Biophys Acta 289(1);19-27. PMID: 4564054

Portalier74: Portalier RC, Robert-Baudouy JM, Nemoz GM (1974). "[Studies of mutations in the uronic isomerase and altronic oxidoreductase structural genes of Escherichia coli K 12 (author's transl)]." Mol Gen Genet 128(4);301-19. PMID: 4594010

Portalier80: Portalier R, Robert-Baudouy J, Stoeber F (1980). "Regulation of Escherichia coli K-12 hexuronate system genes: exu regulon." J Bacteriol 143(3);1095-107. PMID: 6997263

Portalier82: Portalier R, Stoeber F (1982). "D-Mannonate and D-altronate-NAD dehydrogenases from Escherichia coli." Methods Enzymol 89 Pt D;210-8. PMID: 6755169

Pouyssegur71: Pouyssegur J, Stoeber F (1971). "[Study of the common degradative pathway of hexuronates in Escherichia coli K 12. Purification, properties and individuality of 2-keto-3-deoxy-D-gluconnokinase]." Biochimie 53(6);771-81. PMID: 4944816

Showing only 20 references. To show more, press the button "Show all references".


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14B.