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Escherichia coli K-12 substr. MG1655 Pathway: L-homoserine biosynthesis
Inferred from experiment

Pathway diagram: L-homoserine biosynthesis

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for L-homoserine biosynthesis

Superclasses: BiosynthesisAmino Acids BiosynthesisOther Amino Acid Biosynthesis

The homoserine biosynthesis pathway is a three-step pathway that converts L-aspartate into homoserine. Two of the three enzymes that catalyze the first step in this pathway are bifunctional, also serving to catalyze the final step in the pathway.

The final product of this pathway, homoserine, feeds into biosynthetic pathways for both threonine and methionine. Fittingly, this pathway is regulated by the outputs of both of those pathways. metL is repressed by abundant methionine. thrA is regulated by threonine- and isoleucine-dependent attenuation, and both the aspartate kinase and homoserine dehydrogenase activities of ThrA are inhibited by threonine. The middle enzymatic activity, aspartate semialdehyde dehydrogenase, is subject to some form of repression by both methionine and threonine.

Review: Patte, J.-C., Biosynthesis of Threonine and Lysine, Chapter 32 [Neidhardt96]

Superpathways: aspartate superpathway, superpathway of L-lysine, L-threonine and L-methionine biosynthesis I, superpathway of L-threonine biosynthesis, L-homoserine and L-methionine biosynthesis, superpathway of S-adenosyl-L-methionine biosynthesis

Created 14-Oct-1998 by Paley S, SRI International
Last-Curated 16-Aug-2007 by Shearer A, SRI International


Chassagnole01: Chassagnole C, Rais B, Quentin E, Fell DA, Mazat JP (2001). "An integrated study of threonine-pathway enzyme kinetics in Escherichia coli." Biochem J 356(Pt 2);415-23. PMID: 11368768

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Adkins13: Adkins J, Jordan J, Nielsen DR (2013). "Engineering Escherichia coli for renewable production of the 5-carbon polyamide building-blocks 5-aminovalerate and glutarate." Biotechnol Bioeng 110(6);1726-34. PMID: 23296991

Alvarez04: Alvarez E, Ramon F, Magan C, Diez E (2004). "L-cystine inhibits aspartate-beta-semialdehyde dehydrogenase by covalently binding to the essential 135Cys of the enzyme." Biochim Biophys Acta 1696(1);23-9. PMID: 14726201

Angeles89: Angeles TS, Smanik PA, Borders CL, Viola RE (1989). "Aspartokinase-homoserine dehydrogenase I from Escherichia coli: pH and chemical modification studies of the kinase activity." Biochemistry 28(22);8771-7. PMID: 2557908

Angeles90: Angeles TS, Viola RE (1990). "The kinetic mechanisms of the bifunctional enzyme aspartokinase-homoserine dehydrogenase I from Escherichia coli." Arch Biochem Biophys 283(1);96-101. PMID: 2241177

Ataide07: Ataide SF, Wilson SN, Dang S, Rogers TE, Roy B, Banerjee R, Henkin TM, Ibba M (2007). "Mechanisms of resistance to an amino acid antibiotic that targets translation." ACS Chem Biol 2(12);819-27. PMID: 18154269

Bearer78: Bearer CF, Neet KE (1978). "Threonine inhibition of the aspartokinase--homoserine dehydrogenase I of Escherichia coli. Threonine binding studies." Biochemistry 17(17);3512-6. PMID: 28750

Bearer78a: Bearer CF, Neet KE (1978). "Threonine inhibition of the aspartokinase--homoserine dehydrogenase I of Escherichia coli. Stopped-flow kinetics and the cooperativity of inhibition of the homoserine dehydrogenase activity." Biochemistry 17(17);3517-22. PMID: 28751

Bearer78b: Bearer CF, Neet KE (1978). "Threonine inhibition of the aspartokinase--homoserine dehydrogenase I of Escherichia coli. A slow transient and cooperativity of inhibition of the aspartokinase activity." Biochemistry 1978;17(17);3523-30. PMID: 28752

Belfaiza84: Belfaiza J, Fazel A, Muller K, Cohen GN (1984). "E. coli aspartokinase II-homoserine dehydrogenase II polypeptide chain has a triglobular structure." Biochem Biophys Res Commun 123(1);16-20. PMID: 6383377

Biellmann80: Biellmann JF, Eid P, Hirth C (1980). "Affinity labeling of the Escherichia coli aspartate-beta-semialdehyde dehydrogenase with an alkylating coenzyme analogue. Half-site reactivity and competition with the substrate alkylating analogue." Eur J Biochem 1980;104(1);65-9. PMID: 6102911

Biellmann80a: Biellmann JF, Eid P, Hirth C, Jornvall H (1980). "Aspartate-beta-semialdehyde dehydrogenase from Escherichia coli. Purification and general properties." Eur J Biochem 1980;104(1);53-8. PMID: 6102909

Boy72: Boy E, Patte JC (1972). "Multivalent repression of aspartic semialdehyde dehydrogenase in Escherichia coli K-12." J Bacteriol 112(1);84-92. PMID: 4404058

Boy79: Boy E, Borne F, Patte JC (1979). "Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12." Biochimie 61(10);1151-60. PMID: 231461

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Broglie83: Broglie KE, Takahashi M (1983). "Fluorescence studies of threonine-promoted conformational transitions in aspartokinase I using the substrate analogue 2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate." J Biol Chem 1983;258(21);12940-6. PMID: 6313682

Caron12: Caron MP, Bastet L, Lussier A, Simoneau-Roy M, Masse E, Lafontaine DA (2012). "Dual-acting riboswitch control of translation initiation and mRNA decay." Proc Natl Acad Sci U S A 109(50);E3444-53. PMID: 23169642

Cassan75: Cassan M, Boy E, Borne F, Patte JC (1975). "Regulation of the lysine biosynthetic pathway in Escherichia coli K-12: isolation of a cis-dominant constitutive mutant for AK III synthesis." J Bacteriol 123(2);391-9. PMID: 238953

Cassan83: Cassan M, Ronceray J, Patte JC (1983). "Nucleotide sequence of the promoter region of the E. coli lysC gene." Nucleic Acids Res 11(18);6157-66. PMID: 6312411

Cassan86: Cassan M, Parsot C, Cohen GN, Patte JC (1986). "Nucleotide sequence of lysC gene encoding the lysine-sensitive aspartokinase III of Escherichia coli K12. Evolutionary pathway leading to three isofunctional enzymes." J Biol Chem 1986;261(3);1052-7. PMID: 3003049

Chen11b: Chen Z, Rappert S, Sun J, Zeng AP (2011). "Integrating molecular dynamics and co-evolutionary analysis for reliable target prediction and deregulation of the allosteric inhibition of aspartokinase for amino acid production." J Biotechnol 154(4);248-54. PMID: 21609739

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Mon May 2, 2016, biocyc13.