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Escherichia coli K-12 substr. MG1655 Pathway: pentose phosphate pathway (non-oxidative branch)
Inferred from experiment

Pathway diagram: pentose phosphate pathway (non-oxidative branch)

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Genetic Regulation Schematic

Genetic regulation schematic for pentose phosphate pathway (non-oxidative branch)

Superclasses: Generation of Precursor Metabolites and EnergyPentose Phosphate Pathways

The pentose phosphate pathway is one of the three essential pathways of central metabolism. It supplies three of E. coli's 13 precursor metabolites (compounds needed for the biosyntheses): D-ribose-5-phosphate, sedoheptulose-7-phosphate, and erythrose-4-phosphate. Regardless of the carbon source upon which E. coli is growing, some carbon must flow through the pentose phosphate pathway to meet the cell's requirements for these metabolites. In addition this pathway is an important source of reducing equivalents in the form of NADPH, which is also needed for biosyntheses. The pathway begins with one intermediate of glycolysis, glucose-6-phosphate, and ends with the formation of two others, fructose-6-phosphate and D-glyceraldehyde-3-phosphate.

For convenience, the pentose phosphate pathway is commonly divided into its preliminary oxidative portion in which glucose-6-phosphate is oxidized to ribulose-5-phosphate, and its subsequent non-oxidative portion, shown here, in which, through a series of transaldolase and transketolase reactions, ribulose-5-phosphate is converted into fructose-6-phosphate and glyceraldehyde-3-phosphate.

Superpathways: pentose phosphate pathway

Created 04-Apr-1994 by Riley M, Marine Biological Laboratory
Revised 13-Jul-2006 by Ingraham JL, UC Davis

References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Asztalos07: Asztalos P, Parthier C, Golbik R, Kleinschmidt M, Hubner G, Weiss MS, Friedemann R, Wille G, Tittmann K (2007). "Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate." Biochemistry 46(43);12037-52. PMID: 17914867

Aucamp08: Aucamp JP, Martinez-Torres RJ, Hibbert EG, Dalby PA (2008). "A microplate-based evaluation of complex denaturation pathways: structural stability of Escherichia coli transketolase." Biotechnol Bioeng 99(6);1303-10. PMID: 17969139

Avison01: Avison MB, Horton RE, Walsh TR, Bennett PM (2001). "Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media." J Biol Chem 276(29);26955-61. PMID: 11350954

Benov99: Benov L, Fridovich I (1999). "Why superoxide imposes an aromatic amino acid auxotrophy on Escherichia coli. The transketolase connection." J Biol Chem 274(7);4202-6. PMID: 9933617

Binkowski05: Binkowski TA, Joachimiak A, Liang J (2005). "Protein surface analysis for function annotation in high-throughput structural genomics pipeline." Protein Sci 14(12);2972-81. PMID: 16322579

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Chandran03: Chandran SS, Yi J, Draths KM, von Daeniken R, Weber W, Frost JW (2003). "Phosphoenolpyruvate availability and the biosynthesis of shikimic acid." Biotechnol Prog 19(3);808-14. PMID: 12790643

Chauhan96: Chauhan RP, Woodley JM, Powell LW (1996). "In situ product removal from E. coli transketolase-catalyzed biotransformations." Ann N Y Acad Sci 799;545-54. PMID: 8958111

Dalby07: Dalby PA, Aucamp JP, George R, Martinez-Torres RJ (2007). "Structural stability of an enzyme biocatalyst." Biochem Soc Trans 35(Pt 6);1606-9. PMID: 18031275

David70: David J, Wiesmeyer H (1970). "Regulation of ribose metabolism in Escherichia coli. II. Evidence for two ribose-5-phosphate isomerase activities." Biochim Biophys Acta 208(1);56-67. PMID: 4909663

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Domain07: Domain F, Bina XR, Levy SB (2007). "Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR." Mol Microbiol 66(2);383-94. PMID: 17850260

Edwards00: Edwards JS, Palsson BO (2000). "Robustness analysis of the Escherichia coli metabolic network." Biotechnol Prog 16(6);927-39. PMID: 11101318

Essenberg75: Essenberg MK, Cooper RA (1975). "Two ribose-5-phosphate isomerases from Escherichia coli K-12: partial chacterisation of the enzymes and consideration of their possible physiological roles." Eur J Biochem 1975;55:323-332. PMID: 1104357

Flores96: Flores N, Xiao J, Berry A, Bolivar F, Valle F (1996). "Pathway engineering for the production of aromatic compounds in Escherichia coli." Nat Biotechnol 14(5);620-3. PMID: 9630954

Follstad98: Follstad BD, Stephanopoulos G (1998). "Effect of reversible reactions on isotope label redistribution--analysis of the pentose phosphate pathway." Eur J Biochem 252(3);360-71. PMID: 9546650

French96: French C, Ward JM (1996). "Production and modification of E. coli transketolase for large-scale biocatalysis." Ann N Y Acad Sci 799;11-8. PMID: 8958067

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Girgis12: Girgis HS, Harris K, Tavazoie S (2012). "Large mutational target size for rapid emergence of bacterial persistence." Proc Natl Acad Sci U S A 109(31);12740-5. PMID: 22802628

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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