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Escherichia coli K-12 substr. MG1655 Pathway: ADP-L-glycero-β-D-manno-heptose biosynthesis
Inferred from experiment

Pathway diagram: ADP-L-glycero-beta-D-manno-heptose biosynthesis

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Schematic showing all replicons, marked with selected genes

Superclasses: BiosynthesisCarbohydrates BiosynthesisSugars BiosynthesisSugar Nucleotides BiosynthesisADP-sugar Biosynthesis

ADP-L-glycero-β-D-manno-heptose is a precursor for the inner core lipopolysaccharide (LPS) [Kneidinger02]. LPS is a component of the outer membrane of Gram-negative bacteria and consists of lipid A, a core oligosaccharide, and an O-specific polysaccharide (O antigen).

The heptose-1,7-bisphosphate (HBP) intermediate of this pathway is detected within the cytosol of mammalian host cells and induces innate and adaptive immune responses in vivo [Gaudet15].

Reviews: [Valvano02, Raetz02, Brubaker15]

Created 17-Aug-2005 by Keseler I, SRI International


Brubaker15: Brubaker SW, Monack DM (2015). "IMMUNOLOGY. Microbial metabolite triggers antimicrobial defense." Science 348(6240);1207-8. PMID: 26068833

Gaudet15: Gaudet RG, Sintsova A, Buckwalter CM, Leung N, Cochrane A, Li J, Cox AD, Moffat J, Gray-Owen SD (2015). "INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity." Science 348(6240);1251-5. PMID: 26068852

Kneidinger02: Kneidinger B, Marolda C, Graninger M, Zamyatina A, McArthur F, Kosma P, Valvano MA, Messner P (2002). "Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli." J Bacteriol 184(2);363-9. PMID: 11751812

Raetz02: Raetz CR, Whitfield C (2002). "Lipopolysaccharide endotoxins." Annu Rev Biochem 71;635-700. PMID: 12045108

Valvano02: Valvano MA, Messner P, Kosma P (2002). "Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides." Microbiology 148(Pt 7);1979-89. PMID: 12101286

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Brilli04: Brilli M, Fani R (2004). "Molecular evolution of hisB genes." J Mol Evol 58(2);225-37. PMID: 15042344

Brooke96: Brooke JS, Valvano MA (1996). "Biosynthesis of inner core lipopolysaccharide in enteric bacteria identification and characterization of a conserved phosphoheptose isomerase." J Biol Chem 271(7);3608-14. PMID: 8631969

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Coleman79: Coleman WG, Leive L (1979). "Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane." J Bacteriol 139(3);899-910. PMID: 383699

Coleman83: Coleman WG (1983). "The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis." J Biol Chem 258(3);1985-90. PMID: 6337148

Coleman85: Coleman WG, Deshpande KS (1985). "New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide." J Bacteriol 161(3);1209-14. PMID: 3882666

Deacon00: Deacon AM, Ni YS, Coleman WG, Ealick SE (2000). "The crystal structure of ADP-L-glycero-D-mannoheptose 6-epimerase: catalysis with a twist." Structure Fold Des 8(5);453-62. PMID: 10896473

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ding94: Ding L, Seto BL, Ahmed SA, Coleman WG (1994). "Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosugar epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase." J Biol Chem 269(39);24384-90. PMID: 7929099

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Havekes76: Havekes LM, Lugtenberg BJ, Hoekstra WP (1976). "Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide." Mol Gen Genet 146(1);43-50. PMID: 785207

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joloba04: Joloba ML, Clemmer KM, Sledjeski DD, Rather PN (2004). "Activation of the gab operon in an RpoS-dependent manner by mutations that truncate the inner core of lipopolysaccharide in Escherichia coli." J Bacteriol 186(24);8542-6. PMID: 15576807

Karow91: Karow M, Raina S, Georgopoulos C, Fayet O (1991). "Complex phenotypes of null mutations in the htr genes, whose products are essential for Escherichia coli growth at elevated temperatures." Res Microbiol 142(2-3);289-94. PMID: 1656498

Kuznetsova06: Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF (2006). "Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family." J Biol Chem 281(47):36149-61. PMID: 16990279

Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Sat Apr 30, 2016, biocyc14.