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Escherichia coli K-12 substr. MG1655 Pathway: superpathway of purine deoxyribonucleosides degradation

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Locations of Mapped Genes:

Genetic Regulation Schematic: ?

Superclasses: Degradation/Utilization/Assimilation Nucleosides and Nucleotides Degradation
Superpathways

Summary:
E. coli can use all three naturally occurring purine deoxyribonucleosides (deoadenosine, deoxyguanosine, and deoxyinosine) as total sources of carbon and energy. All are cleaved by the same deoD-encoded phosphorylase, yielding the corresponding bases (adenine, guanine and hypoxanthine) and D-deoxyribose-1-phosphate, which is converted to D-deoxyribose-5-phosphate by a mutase that also can utilize D-ribose-1-phosphate as a substrate. A specific aldolase splits D-deoxyribose-5-phosphate, yielding D-glyceraldehyde-3-phosphate, which enters central metabolism through the glycolytic pathway, and acetaldehyde, which must be converted to acetyl-CoA before it can enter central metabolism.

Although E. coli is able to utilize the deoxyribose moiety of deoxyribonucleosides, it is unable to utilize exogenously supplied deoxyribose; however, the closely related organism Salmonella typhimurium can.

The amino nitrogen of adenine and guanine but not the nitrogen atoms in the purine bases can serve as total sources of nitrogen for E. coli.

Review: Linn, E.C.C. Dissimilatory Pathways for Sugars, Polyols, and Carboxylates. E. coli and Salmonella, F.C. Neidhardt, Ed. [Neidhardt96]

Subpathways: purine deoxyribonucleosides degradation I , 2'-deoxy-α-D-ribose 1-phosphate degradation

Credits:
Created 12-Dec-2006 by Ingraham JL , UC Davis


References

Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Acebron09: Acebron SP, Martin I, del Castillo U, Moro F, Muga A (2009). "DnaK-mediated association of ClpB to protein aggregates. A bichaperone network at the aggregate surface." FEBS Lett 583(18);2991-6. PMID: 19698713

Ahmad68: Ahmad SI, Barth PT, Pritchard RH (1968). "Properties of a mutant of Escherichia coli lacking purine nucleoside phosphorylase." Biochim Biophys Acta 161(2);581-3. PMID: 4875425

Ahmad69: Ahmad SI, Pritchard RH (1969). "A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli." Mol Gen Genet 104(4);351-9. PMID: 4904508

Aristarkhov96: Aristarkhov A, Mikulskis A, Belasco JG, Lin EC (1996). "Translation of the adhE transcript to produce ethanol dehydrogenase requires RNase III cleavage in Escherichia coli." J Bacteriol 178(14);4327-32. PMID: 8763968

Bennett03: Bennett EM, Li C, Allan PW, Parker WB, Ealick SE (2003). "Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase." J Biol Chem 278(47);47110-8. PMID: 12937174

Bennett03a: Bennett EM, Anand R, Allan PW, Hassan AE, Hong JS, Levasseur DN, McPherson DT, Parker WB, Secrist JA, Sorscher EJ, Townes TM, Waud WR, Ealick SE (2003). "Designer gene therapy using an Escherichia coli purine nucleoside phosphorylase/prodrug system." Chem Biol 10(12);1173-81. PMID: 14700625

Bertosa14: Bertoša B, Mikleušević G, Wielgus-Kutrowska B, Narczyk M, Hajnic M, Leščić Ašler I, Tomic S, Luic M, Bzowska A (2014). "Homooligomerization is needed for stability: a molecular modelling and solution study of Escherichia coli purine nucleoside phosphorylase." FEBS J 281(7);1860-71. PMID: 24785777

Bezirdzhian86: Bezirdzhian KhO, Kocharian ShM, Akopian ZhI (1986). "[Isolation of the hexameric form of purine nucleoside phosphorylase from E. coli. Comparative study of trimeric and hexameric forms of the enzyme]." Biokhimiia 1986;51(7);1085-92. PMID: 3089333

Bezirdzhian87: Bezirdzhian KhO, Kocharian ShM, Akopian ZhI (1987). "[Hexameric purine nucleoside phosphorylase II from Escherichia coli K-12. Physico-chemical and catalytic properties and stabilization with substrates]." Biokhimiia 1987;52(10);1624-31. PMID: 3122852

BRENDA, 2014: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Burlingame83a: Burlingame R, Chapman PJ (1983). "Stereospecificity in meta-fission catabolic pathways." J Bacteriol 155(1);424-6. PMID: 6345511

Buxton75: Buxton RS (1975). "Genetic analysis of thymidine-resistant and low-thymine-requiring mutants of Escherichia coli K-12 induced by bacteriophage Mu-1." J Bacteriol 121(2);475-84. PMID: 1089630

Bzowska00: Bzowska A, Kulikowska E, Shugar D (2000). "Purine nucleoside phosphorylases: properties, functions, and clinical aspects." Pharmacol Ther 88(3);349-425. PMID: 11337031

Chang91: Chang ZY, Nygaard P, Chinault AC, Kellems RE (1991). "Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function." Biochemistry 30(8);2273-80. PMID: 1998686

Chen91: Chen YM, Lin EC (1991). "Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli." J Bacteriol 1991;173(24);8009-13. PMID: 1744060

Clark80: Clark DP, Cronan JE (1980). "Acetaldehyde coenzyme A dehydrogenase of Escherichia coli." J Bacteriol 144(1);179-84. PMID: 6998946

Clark89: Clark DP (1989). "The fermentation pathways of Escherichia coli." FEMS Microbiol Rev 1989;5(3);223-34. PMID: 2698228

Dailly00: Dailly YP, Bunch P, Clark DP (2000). "Comparison of the fermentative alcohol dehydrogenases of Salmonella typhimurium and Escherichia coli." Microbios 103(406);179-96. PMID: 11131810

Dale67: Dale B, Greenberg GR (1967). "Genetic mapping of a mutation in Escherichia coli showing reduced activity of thymidine phosphorylase." J Bacteriol 94(3);778-9. PMID: 5340684

Dandanell05: Dandanell G, Szczepanowski RH, Kierdaszuk B, Shugar D, Bochtler M (2005). "Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene." J Mol Biol 348(1);113-25. PMID: 15808857

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14B.