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Escherichia coli K-12 substr. MG1655 Protein: putative transport complex, ABC superfamily
Inferred by computational analysis

Subunit composition of putative transport complex, ABC superfamily = [YbbP][YbbA]
         putative transport protein, ABC superfamily - membrane component = YbbP (summary available)
         putative transport protein, ABC superfamily - ATP-binding component = YbbA (summary available)

Summary:
YbbA and YbbP are uncharacterized members of the ABC superfamily of transporters [Saurin99]. YbbA is the putative ATP binding component. YbbP may be the membrane component. Based on sequence similarity, these proteins may work together as part of an ATP dependent metabolite uptake system. The genes ybbA and ybbP are probably located within a single operon.

Gene-Reaction Schematic

Gene-Reaction Schematic


Subunit of putative transport complex, ABC superfamily: putative transport protein, ABC superfamily - membrane component

Synonyms: YbbP

Gene: ybbP Accession Numbers: G6270 (EcoCyc), b0496, ECK0490

Locations: inner membrane

Sequence Length: 804 AAs

Molecular Weight: 89.332 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases

Unification Links: DIP:DIP-11324N, EcoliWiki:b0496, Mint:MINT-1286702, Pride:P77504, Protein Model Portal:P77504, RefSeq:NP_415029, UniProt:P77504

Relationship Links: InterPro:IN-FAMILY:IPR003838, Pfam:IN-FAMILY:PF02687

Summary:
YbbP is predicted to be the membrane component of an ABC superfamily metabolite uptake transporter.

Essentiality data for ybbP knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of putative transport complex, ABC superfamily: putative transport protein, ABC superfamily - ATP-binding component

Synonyms: YbbA

Gene: ybbA Accession Numbers: EG11657 (EcoCyc), b0495, ECK0489

Locations: inner membrane, cytosol

Sequence Length: 228 AAs

Molecular Weight: 24.533 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0008152 - metabolic process [GOA01a]
Molecular Function:
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Cellular Component:
GO:0005737 - cytoplasm []
GO:0005829 - cytosol []
Inferred by computational analysisGO:0005886 - plasma membrane [DiazMejia09]

MultiFun Terms: transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b0495, Mint:MINT-1283721, ModBase:P0A9T8, Pride:P0A9T8, Protein Model Portal:P0A9T8, RefSeq:NP_415028, SMR:P0A9T8, UniProt:P0A9T8

Relationship Links: InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, Pfam:IN-FAMILY:PF00005, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

Summary:
YbbA is an ATP-binding component of a predicted ABC superfamily metabolite uptake transporter.

Essentiality data for ybbA knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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