Escherichia coli K-12 substr. MG1655 Polypeptide: lysine / arginine / ornithine ABC transporter - periplasmic binding protein

Gene: argT Accession Numbers: EG10072 (EcoCyc), b2310, ECK2304

Regulation Summary Diagram

Regulation summary diagram for argT

Component of: lysine / arginine / ornithine ABC transporter (extended summary available)

Gene Citations: [Zimmer00]

Locations: periplasmic space

Map Position: [2,425,031 <- 2,425,813] (52.27 centisomes, 188°)
Length: 783 bp / 260 aa

Molecular Weight of Polypeptide: 27.992 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007621, CGSC:18529, DIP:DIP-9148N, EchoBASE:EB0070, EcoGene:EG10072, EcoliWiki:b2310, ModBase:P09551, OU-Microarray:b2310, PortEco:argT, PR:PRO_000022136, Pride:P09551, Protein Model Portal:P09551, RefSeq:NP_416813, RegulonDB:EG10072, SMR:P09551, String:511145.b2310, Swiss-Model:P09551, UniProt:P09551

Relationship Links: InterPro:IN-FAMILY:IPR001638, InterPro:IN-FAMILY:IPR005768, InterPro:IN-FAMILY:IPR018313, Pfam:IN-FAMILY:PF00497, Prosite:IN-FAMILY:PS01039, Smart:IN-FAMILY:SM00062

Gene-Reaction Schematic

Gene-Reaction Schematic

Genetic Regulation Schematic

Genetic regulation schematic for argT

GO Terms:
Biological Process:
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a]
Molecular Function:
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Inferred by computational analysisGO:0015426 - polar-amino acid-transporting ATPase activity []
Cellular Component:
Inferred by computational analysisGO:0030288 - outer membrane-bounded periplasmic space [GOA01a]
Inferred by computational analysisGO:0042597 - periplasmic space [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolismbiosynthesis of building blocksamino acidsarginine
metabolismbiosynthesis of building blocksamino acidslysine
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Essentiality data for argT knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: lysine / arginine / ornithine ABC transporter

Subunit composition of lysine / arginine / ornithine ABC transporter = [HisQ][HisP]2[HisM][ArgT]
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisQ
         lysine / arginine / ornithine ABC transporter / histidine ABC transporter - ATP binding subunit = HisP
         lysine/arginine/ornithine ABC transporter / histidine ABC transporter - membrane subunit = HisM
         lysine / arginine / ornithine ABC transporter - periplasmic binding protein = ArgT

hisP and hisMQ encode the ATP-binding component and two integral membrane components, respectively, of both the lysine/arginine/ornithine ABC transporter and the histidine ABC transporter of E. coli.

argT is a homolog of argT of Salmonella typhimurium which encodes a lysing/arginine/ornithine periplasmic binding protein [Nonet87]. The substrate specificities of ArgT and HisJ, the periplasmic histidine-binding protein, are believed to have diverged after a gene duplication event while the reliance upon HisPMQ for transport has been conserved [Higgins81].

Expression of argT is decreased after four hours of Zn(II) stress [Easton06]. argT is also repressed in response to stationary phase supernatant of autoinducer-2 deficient cells, suggesting another quorum sensing system is involved in controlling argT expression [Ren04a]. In clpP and clpA protease mutants, late stationary phase induction of argT does not occur [Weichart03]. Expression increased in early response to glucose limitation [Wick01], after ten hours of nitrogen starvation [Kabir04a], and in a glnL(Up) mutant in which NtrC-activated genes are overexpressed compared to a ntrC null mutant [Zimmer00].

Enzymatic reaction of: transport of L-ornithine (lysine / arginine / ornithine ABC transporter)

Inferred by computational analysis

Transport reaction diagram for transport of L-ornithine

Enzymatic reaction of: transport of L-arginine (lysine / arginine / ornithine ABC transporter)

Inferred by computational analysis

Transport reaction diagram for transport of L-arginine

Enzymatic reaction of: transport of L-lysine (lysine / arginine / ornithine ABC transporter)

Inferred by computational analysis

Transport reaction diagram for transport of L-lysine

Sequence Features

Protein sequence of lysine / arginine / ornithine ABC transporter - periplasmic binding protein with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 22
Inferred from experiment[Link97]
Sequence-Conflict 23
Inferred by curator[Nonet87, UniProt15]
UniProt: (in Ref. 4; AAA23971).
Chain 23 -> 260
Author statement[UniProt15]
UniProt: Lysine/arginine/ornithine-binding periplasmic protein.
Pfam PF00497 28 -> 254
Inferred by computational analysis[Finn14]
SBP_bac_3 : Bacterial extracellular solute-binding proteins, family 3
Disulfide-Bond-Site 60, 67
Inferred by computational analysis[UniProt15]
Sequence-Conflict 86 -> 87
Inferred by curator[Nonet87, UniProt15]
UniProt: (in Ref. 4).
Sequence-Conflict 95
Inferred by curator[Nonet87, UniProt15]
UniProt: (in Ref. 4).
Sequence-Conflict 200 -> 201
Inferred by curator[UniProt15]
UniProt: (in Ref. 5; AAA85768).
Sequence-Conflict 213
Inferred by curator[UniProt15]
UniProt: (in Ref. 5; AAA85768).
Sequence-Conflict 237 -> 239
Inferred by curator[UniProt15]
UniProt: (in Ref. 5; AAA85768).

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Gene local context diagram

Transcription Units

Transcription-unit diagram

Transcription-unit diagram


3/2/1998 (pkarp) Merged genes G388/b2310 and EG10072/argT
1/26/1998 (pkarp) Merged genes G7989/argT and EG10072/argT


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Easton06: Easton JA, Thompson P, Crowder MW (2006). "Time-dependent translational response of E. coli to excess Zn(II)." J Biomol Tech 17(5);303-7. PMID: 17122063

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Higgins81: Higgins CF, Ames GF (1981). "Two periplasmic transport proteins which interact with a common membrane receptor show extensive homology: complete nucleotide sequences." Proc Natl Acad Sci U S A 78(10);6038-42. PMID: 6273842

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kabir04a: Kabir MS, Sagara T, Oshima T, Kawagoe Y, Mori H, Tsunedomi R, Yamada M (2004). "Effects of mutations in the rpoS gene on cell viability and global gene expression under nitrogen starvation in Escherichia coli." Microbiology 150(Pt 8);2543-53. PMID: 15289551

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

Nonet87: Nonet ML, Marvel CC, Tolan DR (1987). "The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons." J Biol Chem 262(25);12209-17. PMID: 3040734

Ren04a: Ren D, Bedzyk LA, Ye RW, Thomas SM, Wood TK (2004). "Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli." Appl Environ Microbiol 70(4);2038-43. PMID: 15066794

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Weichart03: Weichart D, Querfurth N, Dreger M, Hengge-Aronis R (2003). "Global role for ClpP-containing proteases in stationary-phase adaptation of Escherichia coli." J Bacteriol 185(1);115-25. PMID: 12486047

Wick01: Wick LM, Quadroni M, Egli T (2001). "Short- and long-term changes in proteome composition and kinetic properties in a culture of Escherichia coli during transition from glucose-excess to glucose-limited growth conditions in continuous culture and vice versa." Environ Microbiol 3(9);588-99. PMID: 11683869

Zimmer00: Zimmer DP, Soupene E, Lee HL, Wendisch VF, Khodursky AB, Peter BJ, Bender RA, Kustu S (2000). "Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation." Proc Natl Acad Sci U S A 97(26);14674-9. PMID: 11121068

Other References Related to Gene Regulation

Barrios99: Barrios H, Valderrama B, Morett E (1999). "Compilation and analysis of sigma(54)-dependent promoter sequences." Nucleic Acids Res 27(22);4305-13. PMID: 10536136

MedinaRivera11: Medina-Rivera A, Abreu-Goodger C, Thomas-Chollier M, Salgado H, Collado-Vides J, van Helden J (2011). "Theoretical and empirical quality assessment of transcription factor-binding motifs." Nucleic Acids Res 39(3);808-24. PMID: 20923783

Reitzer01: Reitzer L, Schneider BL (2001). "Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli." Microbiol Mol Biol Rev 65(3);422-44, table of contents. PMID: 11528004

Schmitz87: Schmitz G, Durre P, Mullenbach G, Ames GF (1987). "Nitrogen regulation of transport operons: analysis of promoters argTr and dhuA." Mol Gen Genet 209(2);403-7. PMID: 3118148

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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