Escherichia coli K-12 substr. MG1655 Protein: heat shock protein, hemimethylated DNA-binding protein
Inferred from experiment

Gene: hspQ Accession Numbers: G6500 (EcoCyc), b0966, ECK0957

Synonyms: yccV

Regulation Summary Diagram

Regulation summary diagram for hspQ

Subunit composition of heat shock protein, hemimethylated DNA-binding protein = [HspQ]2
         heat shock protein, hemimethylated DNA-binding protein = HspQ

HspQ binds to hemimethylated DNA at oriC [dAlencon03]. The HspQ protein forms dimers and higher order multimers in solution [Shimuta04].

A hspQ mutation causes overproduction of DnaA and suppresses the temperature sensitivity of dnaA mutants [dAlencon03, Shimuta04]. The dnaA alleles dnaA508 and dnaA167 are sensitive to overexpression of HspQ. HspQ protein stimulates the degradation of the DnaA508 mutant protein, but not DnaA46 [Shimuta04].

hspQ transcription depends on the heat shock sigma factor σ32 [Shimuta04].

Locations: cytosol

Map Position: [1,028,404 <- 1,028,721] (22.16 centisomes, 80°)
Length: 318 bp / 105 aa

Molecular Weight of Polypeptide: 11.779 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003269, DIP:DIP-48155N, EchoBASE:EB3488, EcoGene:EG13724, EcoliWiki:b0966, OU-Microarray:b0966, PortEco:hspQ, PR:PRO_000022936, Protein Model Portal:P0AB20, RefSeq:NP_415486, RegulonDB:G6500, SMR:P0AB20, String:511145.b0966, UniProt:P0AB20

Relationship Links: InterPro:IN-FAMILY:IPR011722, InterPro:IN-FAMILY:IPR022866, PDB:Structure:1VBV, Pfam:IN-FAMILY:PF08755, Smart:IN-FAMILY:SM00992

Gene-Reaction Schematic

Gene-Reaction Schematic

GO Terms:
Biological Process:
Inferred from experimentGO:0045892 - negative regulation of transcription, DNA-templated [dAlencon03]
Inferred by computational analysisGO:0009408 - response to heat [GOA06]
Molecular Function:
Inferred from experimentGO:0008327 - methyl-CpG binding [dAlencon03]
Inferred by computational analysisGO:0003677 - DNA binding [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005737 - cytoplasm [UniProtGOA11, UniProtGOA11a, GOA06]

MultiFun Terms: information transferDNA related

Essentiality data for hspQ knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of heat shock protein, hemimethylated DNA-binding protein with features indicated

Feature Class Location Citations Comment
Pfam PF08755 5 -> 96
Inferred by computational analysis[Finn14]
YccV-like : Hemimethylated DNA-binding protein YccV like [More...]

Gene Local Context (not to scale -- see Genome Browser for correct scale)

Transcription Unit

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06].
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

dAlencon03: d'Alencon E, Taghbalout A, Bristow C, Kern R, Aflalo R, Kohiyama M (2003). "Isolation of a new hemimethylated DNA binding protein which regulates dnaA gene expression." J Bacteriol 185(9);2967-71. PMID: 12700277

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Shimuta04: Shimuta TR, Nakano K, Yamaguchi Y, Ozaki S, Fujimitsu K, Matsunaga C, Noguchi K, Emoto A, Katayama T (2004). "Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli." Genes Cells 9(12);1151-66. PMID: 15569148

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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