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Escherichia coli K-12 substr. MG1655 Protein: ArsR DNA-binding transcriptional repressor



Gene: arsR Accession Numbers: EG12235 (EcoCyc), b3501, ECK3486

Synonyms: arsE

Regulation Summary Diagram: ?

Component of:
ArsR-antimonite
ArsR-arsenite

Subunit composition of ArsR DNA-binding transcriptional repressor = [ArsR]2

Summary:
ArsR negatively controls the expression of the genes involved in arsenical and antimonite metals resistance whose expression is induced in the presence of these metals [Carlin95, Diorio95, Cai96, Xu96a]. This protein is autoregulated; arsR is the first gene in the arsRBC operon that it regulates [Diorio95].

ArsR belongs to the ArsR/SmtB family of transcriptional regulators that respond to a variety of metals [Busenlehner03]. ArsR has a helix-turn-helix motif for DNA binding, a metal-binding site [Shi94], and a dimerization domain [Xu97a]. The inducer-binding sites for the members of this family appear to have arisen by convergent evolution [Qin07]. In ArsR the inducer-binding site contains three cysteine residues that bind arsenite and antimonite specifically and with high affinity [Shi94, Shi96].

Dimerization of ArsR is required for DNA binding and its ability to act as a transcriptional repressor [Xu97a]. The dimer recognizes and binds to a 12-2-12 inverted repeat [Busenlehner03, Xu96a], but the binding of arsenic or antimonite to ArsR causes a conformational change in it, leading to dissociation from DNA and hence derepression [Shi96].

The chromosomal ArsR shows 75% identity to ArsR of Escherichia coli plasmid R773 and 26% identity to staphylococcal plasmid pI258 and pSX267 ArsR proteins [Carlin95].

ArsR when overexpressed has the capacity to remove arsenic species from contaminated water [Kostal04, Yang13a].

ArsR: homologous to "arsenate inducibility regulator" [Diorio95].

Review: [Busenlehner03].

Locations: cytosol

Map Position: [3,646,551 -> 3,646,904] (78.59 centisomes)
Length: 354 bp / 117 aa

Molecular Weight of Polypeptide: 13.253 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011435 , CGSC:35993 , DIP:DIP-9163N , EchoBASE:EB2147 , EcoGene:EG12235 , EcoliWiki:b3501 , Mint:MINT-1525524 , ModBase:P37309 , OU-Microarray:b3501 , PortEco:arsR , PR:PRO_000022156 , Protein Model Portal:P37309 , RefSeq:NP_417958 , RegulonDB:EG12235 , SMR:P37309 , String:511145.b3501 , UniProt:P37309

Relationship Links: InterPro:IN-FAMILY:IPR001845 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR018334 , Pfam:IN-FAMILY:PF01022 , Prints:IN-FAMILY:PR00778 , Prosite:IN-FAMILY:PS00846 , Prosite:IN-FAMILY:PS50987 , Smart:IN-FAMILY:SM00418

In Reactions of unknown directionality:

Not in pathways:
ArsR + antimonite ion = ArsR-antimonite
ArsR + arsenite = ArsR-arsenite

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Xu96a]
GO:0006355 - regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Cai96]
GO:0046685 - response to arsenic-containing substance Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Diorio95]
Molecular Function: GO:0003677 - DNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Xu97a]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005622 - intracellular Inferred by computational analysis [GOA01a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes protection drug resistance/sensitivity
information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level repressor

DNA binding site length: 24 base-pairs

Symmetry: Inverted Repeat

Consensus DNA Binding Sequence: TAAGTCAtAtAtgTtTtTGACTTA

Regulated Transcription Units (1 total): ?

Notes:

Essentiality data for arsR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Created 19-Oct-2008 by Gama-Castro S , UNAM
Last-Curated ? 09-Dec-2008 by Gama-Castro S , UNAM


Subunit of: ArsR-antimonite

Subunit composition of ArsR-antimonite = [ArsR][antimonite ion]

Sequence Length: 117 AAs

In Reactions of unknown directionality:

Not in pathways:
ArsR + antimonite ion = ArsR-antimonite

MultiFun Terms: cell processes protection drug resistance/sensitivity
information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level repressor


Subunit of: ArsR-arsenite

Subunit composition of ArsR-arsenite = [ArsR][arsenite]

Sequence Length: 117 AAs

In Reactions of unknown directionality:

Not in pathways:
ArsR + arsenite = ArsR-arsenite

MultiFun Terms: cell processes protection drug resistance/sensitivity
information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level repressor


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 92
[UniProt09]
UniProt: HTH arsR-type;
DNA-Binding-Region 33 -> 52
[UniProt10]
UniProt: H-T-H motif; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3501 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12235; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Busenlehner03: Busenlehner LS, Pennella MA, Giedroc DP (2003). "The SmtB/ArsR family of metalloregulatory transcriptional repressors: Structural insights into prokaryotic metal resistance." FEMS Microbiol Rev 27(2-3);131-43. PMID: 12829264

Cai96: Cai J, DuBow MS (1996). "Expression of the Escherichia coli chromosomal ars operon." Can J Microbiol 1996;42(7);662-71. PMID: 8764681

Carlin95: Carlin A, Shi W, Dey S, Rosen BP (1995). "The ars operon of Escherichia coli confers arsenical and antimonial resistance." J Bacteriol 1995;177(4);981-6. PMID: 7860609

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Diorio95: Diorio C, Cai J, Marmor J, Shinder R, DuBow MS (1995). "An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria." J Bacteriol 1995;177(8);2050-6. PMID: 7721697

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kostal04: Kostal J, Yang R, Wu CH, Mulchandani A, Chen W (2004). "Enhanced arsenic accumulation in engineered bacterial cells expressing ArsR." Appl Environ Microbiol 70(8);4582-7. PMID: 15294789

Qin07: Qin J, Fu HL, Ye J, Bencze KZ, Stemmler TL, Rawlings DE, Rosen BP (2007). "Convergent evolution of a new arsenic binding site in the ArsR/SmtB family of metalloregulators." J Biol Chem 282(47);34346-55. PMID: 17897948

Shi94: Shi W, Wu J, Rosen BP (1994). "Identification of a putative metal binding site in a new family of metalloregulatory proteins." J Biol Chem 269(31);19826-9. PMID: 8051064

Shi96: Shi W, Dong J, Scott RA, Ksenzenko MY, Rosen BP (1996). "The role of arsenic-thiol interactions in metalloregulation of the ars operon." J Biol Chem 271(16);9291-7. PMID: 8621591

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Xu96a: Xu C, Shi W, Rosen BP (1996). "The chromosomal arsR gene of Escherichia coli encodes a trans-acting metalloregulatory protein." J Biol Chem 1996;271(5);2427-32. PMID: 8576202

Xu97a: Xu C, Rosen BP (1997). "Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli." J Biol Chem 272(25);15734-8. PMID: 9188467

Yang13a: Yang T, Liu JW, Gu C, Chen ML, Wang JH (2013). "Expression of arsenic regulatory protein in Escherichia coli for selective accumulation of methylated arsenic species." ACS Appl Mater Interfaces 5(7);2767-72. PMID: 23484908


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc11.