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discounted EARLY registration ends Dec 31, 2014
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Protein: predicted carbamoyltransferase



Gene: ygeW Accession Numbers: G7489 (EcoCyc), b2870, ECK2866

Regulation Summary Diagram: ?

Subunit composition of predicted carbamoyltransferase = [YgeW]3
         predicted carbamoyltransferase = YgeW

Summary:
YgeW was suggested to function as an oxamate transcarbamoylase in a predicted purine catabolic pathway [Xi00].

A crystal structure of YgeW has been solved at 2 Å resolution, identifying the protein as a member of the transcarbamylase family. The protein was assayed for transcarbamylase activity with a variety of potential substrates including allantoin and oxamate, but no activity was detected [Li11b].

Citations: [ParadisBleau14]

Locations: cytosol

Map Position: [3,004,284 -> 3,005,474] (64.75 centisomes)
Length: 1191 bp / 396 aa

Molecular Weight of Polypeptide: 44.187 kD (from nucleotide sequence)

Molecular Weight of Multimer: 148.0 kD (experimental) [Li11b]

Unification Links: ASAP:ABE-0009425 , EchoBASE:EB2865 , EcoGene:EG13053 , EcoliWiki:b2870 , ModBase:Q46803 , OU-Microarray:b2870 , PortEco:ygeW , Protein Model Portal:Q46803 , RefSeq:YP_026185 , RegulonDB:G7489 , SMR:Q46803 , String:511145.b2870 , UniProt:Q46803

Relationship Links: InterPro:IN-FAMILY:IPR002082 , InterPro:IN-FAMILY:IPR006130 , InterPro:IN-FAMILY:IPR006131 , InterPro:IN-FAMILY:IPR006132 , InterPro:IN-FAMILY:IPR017702 , PDB:Structure:3Q98 , Pfam:IN-FAMILY:PF00185 , Pfam:IN-FAMILY:PF02729 , Prints:IN-FAMILY:PR00100 , Prints:IN-FAMILY:PR00101

In Paralogous Gene Group: 84 (4 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process Inferred by computational analysis [GOA01a]
GO:0006520 - cellular amino acid metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0004070 - aspartate carbamoyltransferase activity Inferred by computational analysis [GOA01a]
GO:0016597 - amino acid binding Inferred by computational analysis [GOA01a]
GO:0016743 - carboxyl- or carbamoyltransferase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs

Essentiality data for ygeW knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Created 10-Jun-2011 by Keseler I , SRI International
Last-Curated ? 10-Jun-2011 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Suzanne Paley on Thu Oct 21, 2004:
Position updated based on U00096.2 release of genome
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Li11b: Li Y, Jin Z, Yu X, Allewell NM, Tuchman M, Shi D (2011). "The ygeW encoded protein from Escherichia coli is a knotted ancestral catabolic transcarbamylase." Proteins 79(7);2327-34. PMID: 21557323

ParadisBleau14: Paradis-Bleau C, Kritikos G, Orlova K, Typas A, Bernhardt TG (2014). "A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism." PLoS Genet 10(1);e1004056. PMID: 24391520

Xi00: Xi H, Schneider BL, Reitzer L (2000). "Purine catabolism in Escherichia coli and function of xanthine dehydrogenase in purine salvage." J Bacteriol 182(19);5332-41. PMID: 10986234


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc11.